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Genome size, cytogenetic data and transferability of EST-SSRs markers in wild and cultivated species of the genus Theobroma L. (Byttnerioideae, Malvaceae)

da Silva Rangeline Azevedo, Souza Gustavo, Lemos Lívia Santos Lima, Lopes Uilson Vanderlei, Patrocinio Nara Geórgia Ribeiro Braz, Alves Rafael Moyses, Marcellino Lucilia Helena, Clément Didier, Micheli Fabienne, Gramacho Karina Peres. 2017. Genome size, cytogenetic data and transferability of EST-SSRs markers in wild and cultivated species of the genus Theobroma L. (Byttnerioideae, Malvaceae). PloS One, 12 (2):e0170799, 15 p.

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Url - jeu de données - Entrepôt autre : https://figshare.com/articles/Genome_size_cytogenetic_data_and_transferability_of_EST-SSRs_markers_in_wild_and_cultivated_species_of_the_genus_i_Theobroma_i_L_Byttnerioideae_Malvaceae_/4641886

Quartile : Q1, Sujet : MULTIDISCIPLINARY SCIENCES

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Psychologie-éthologie-ergonomie; Staps

Résumé : The genus Theobroma comprises several trees species native to the Amazon. Theobroma cacao L. plays a key economic role mainly in the chocolate industry. Both cultivated and wild forms are described within the genus. Variations in genome size and chromosome number have been used for prediction purposes including the frequency of interspecific hybridization or inference about evolutionary relationships. In this study, the nuclear DNA content, karyotype and genetic diversity using functional microsatellites (EST-SSR) of seven Theobroma species were characterized. The nuclear content of DNA for all analyzed Theobroma species was 1C = ~ 0.46 pg. These species presented 2n = 20 with small chromosomes and only one pair of terminal heterochromatic bands positively stained (CMA+/DAPI− bands). The small size of Theobroma ssp. genomes was equivalent to other Byttnerioideae species, suggesting that the basal lineage of Malvaceae have smaller genomes and that there was an expansion of 2C values in the more specialized family clades. A set of 20 EST-SSR primers were characterized for related species of Theobroma, in which 12 loci were polymorphic. The polymorphism information content (PIC) ranged from 0.23 to 0.65, indicating a high level of information per locus. Combined results of flow cytometry, cytogenetic data and EST-SSRs markers will contribute to better describe the species and infer about the evolutionary relationships among Theobroma species. In addition, the importance of a core collection for conservation purposes is highlighted.

Mots-clés Agrovoc : Theobroma, Theobroma cacao, plante de culture, plante sauvage, génome, cytogénétique, marqueur génétique, microsatellite, cytométrie en flux (cellules), identification, Theobroma grandiflorum

Mots-clés géographiques Agrovoc : Bahia, Para

Mots-clés complémentaires : Theobroma bicolor, Theobroma obovatum, Theobroma microcarpum, Theobroma speciosum, Theobroma subincanum, ssr

Classification Agris : F30 - Génétique et amélioration des plantes
F60 - Physiologie et biochimie végétale
U30 - Méthodes de recherche
F70 - Taxonomie végétale et phytogéographie

Champ stratégique Cirad : Axe 1 (2014-2018) - Agriculture écologiquement intensive

Auteurs et affiliations

  • da Silva Rangeline Azevedo, UESC (BRA)
  • Souza Gustavo, UFPE (BRA)
  • Lemos Lívia Santos Lima, CEPEC (BRA)
  • Lopes Uilson Vanderlei, CEPEC (BRA)
  • Patrocinio Nara Geórgia Ribeiro Braz, CEPLAC (BRA)
  • Alves Rafael Moyses, EMBRAPA (BRA)
  • Marcellino Lucilia Helena, EMBRAPA (BRA)
  • Clément Didier, CIRAD-BIOS-UMR AGAP (BRA) ORCID: 0000-0002-7602-6472
  • Micheli Fabienne, CIRAD-BIOS-UMR AGAP (BRA)
  • Gramacho Karina Peres, CEPEC (BRA)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/583605/)

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