Differential fine-tuning of gene expression regulation in coffee leaves by CcDREB1D promoter haplotypes under water deficit

Costa Alves Gabriel Sergio, Ferreira Torres Luana, Dechamp Eveline, Breitler Jean-Christophe, Joët Thierry, Gatineau Frédéric, Carvalho Andrade Alan, Bertrand Benoît, Marraccini Pierre, Etienne Hervé. 2017. Differential fine-tuning of gene expression regulation in coffee leaves by CcDREB1D promoter haplotypes under water deficit. Journal of Experimental Botany, 68 (11) : 3017-3031.

https://doi.org/10.1093/jxb/erx166

Article de revue ; Article de recherche ; Article de revue à facteur d'impact

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Quartile : Q1, Sujet : PLANT SCIENCES

Résumé : Despite the importance of the DREB1D gene (also known as CBF4) in plant responses to water deficit and cold stress, studies analysing its regulation by transgenic approaches are lacking. In the current work, a functional study of three CcDREB1D promoter haplotypes (named HP15, HP16 and HP17) isolated from drought-tolerant and drought-sensitive clones of Coffea canephora was carried out in plants of C. arabica stably transformed by Agrobacterium tumefaciens by analysing their ability to regulate the expression of the uidA reporter gene in response to water deficit mimicked by polyethylene glycol (−2.0 MPa) and low relative humidity treatments. A deletion analysis of their corresponding 5′-upstream regions revealed increased specificity of β-glucuronidase activity in the polyethylene glycol and low relative humidity treatments, with high expression in leaf mesophyll and guard cells in full-length constructs. RT-qPCR assays also revealed that the HP16 haplotype (specific to clone tolerant to water deficit) had stronger and earlier activity compared with the HP15 and HP17 haplotypes. As most of the cis-regulatory elements involved in ABA-dependent and -independent networks, tissue specificity and light regulation are common to these haplotypes, we propose that their organization, as well as the nucleic acid polymorphisms present outside these boxes, may play a role in modulating activities of DREB1D promoters in guard cells.

Mots-clés Agrovoc : Coffea canephora, transformation génétique, tolérance à la sécheresse, expression des gènes, génie génétique, résistance à la sécheresse, résistance génétique, Agrobacterium tumefaciens, régulation physiologique

Mots-clés géographiques Agrovoc : Brésil, France

Mots-clés complémentaires : Promoteur

Mots-clés libres : Coffee, DREB1D gene, Genetic transformation, Promoter haplotypes, UidA reporter gene, Water deficit

Classification Agris : F30 - Génétique et amélioration des plantes
F60 - Physiologie et biochimie végétale
F61 - Physiologie végétale - Nutrition

Champ stratégique Cirad : Axe 1 (2014-2018) - Agriculture écologiquement intensive

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