Next-generation sequencing accelerates crop gene discovery

le Nguyen Khanh, Grondin Alexandre, Courtois Brigitte, Gantet Pascal. 2019. Next-generation sequencing accelerates crop gene discovery. Trends in Plant Science, 24 (3) : 263-274.

Quartile : Outlier, Sujet : PLANT SCIENCES

Résumé : The identification and isolation of genes underlying quantitative trait loci (QTLs) associated with agronomic traits in crops have been recently accelerated thanks to next-generation sequencing (NGS)-based technologies combined with plant genetics. With NGS, various revisited genetic approaches, which benefited from higher marker density, have been elaborated. These approaches improved resolution in QTL position and assisted in determining functional causative variations in genes. Examples of QTLs/genes associated with agronomic traits in crops and identified using different strategies based on whole-genome sequencing (WGS)/whole-genome resequencing (WGR) or RNA-seq are presented and discussed in this review. More specifically, we summarize and illustrate how NGS boosted bulk-segregant analysis (BSA), expression profiling, and the construction of polymorphism databases to facilitate the detection of QTLs and causative genes.

Mots-clés Agrovoc : locus des caractères quantitatifs, marqueur génétique, caractère agronomique, génome, caractéristique génomique, séquence d'arn, polymorphisme génétique

Classification Agris : F30 - Génétique et amélioration des plantes
F01 - Culture des plantes

Champ stratégique Cirad : CTS 2 (2019-) - Transitions agroécologiques

Auteurs et affiliations

• le Nguyen Khanh, Université de Montpellier (FRA)
• Grondin Alexandre, UM2 (FRA)
• Courtois Brigitte, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0003-2118-7102
• Gantet Pascal, UM2 (FRA) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/590812/)

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