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Exploring the diversity of Poaceae infecting mastreviruses on Reunion Island using a viral metagenomicsbased approach

Claverie Sohini, Ouattara Alassane, Hoareau Murielle, Filloux Denis, Varsani Arvind, Roumagnac Philippe, Martin Darren Patrick, Lett Jean-Michel, Lefeuvre Pierre. 2019. Exploring the diversity of Poaceae infecting mastreviruses on Reunion Island using a viral metagenomicsbased approach. Scientific Reports, 9:12716, 11 p.

Journal article ; Article de recherche ; Article de revue à facteur d'impact Revue en libre accès total
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Quartile : Q1, Sujet : MULTIDISCIPLINARY SCIENCES

Abstract : Mostly found in Africa and its surrounding islands, African streak viruses (AfSV) represent the largest group of known mastreviruses. Of the thirteen AfSV species that are known to infect either cultivated or wild Poaceae plant species, six have been identified on Reunion Island. To better characterize AfSV diversity on this island, we undertook a survey of a small agroecosystem using a new metagenomics-based approach involving rolling circle amplification with random PCR amplification tagging (RCA-RA-PCR), high-throughput sequencing (Illumina HiSeq) and the mastrevirus reads classification using phylogenetic placement. Mastreviruses that likely belong to three new species were discovered and full genome sequences of these were determined by Sanger sequencing. The geminivirus-focused metagenomics approach we applied in this study was useful in both the detection of known and novel mastreviruses. The results confirm that Reunion Island is indeed a hotspot of AfSV diversity and that many of the mastrevirus species have likely been introduced multiple times. Applying a similar approach in other natural and agricultural environments should yield sufficient detail on the composition and diversity of geminivirus communities to precipitate major advances in our understanding of the ecology and the evolutionary history of this important group of viruses.

Mots-clés Agrovoc : Poaceae, Géminivirus striure du maïs, Maladie des plantes, Variation génétique, PCR

Mots-clés géographiques Agrovoc : Réunion

Mots-clés complémentaires : Maize streak virus, AfSV

Classification Agris : H20 - Plant diseases

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Agence(s) de financement européenne(s) : European Regional Development Fund, erd

Auteurs et affiliations

  • Claverie Sohini, CIRAD-BIOS-UMR PVBMT (REU)
  • Ouattara Alassane, CIRAD-BIOS-UMR BGPI (FRA)
  • Hoareau Murielle, CIRAD-BIOS-UMR PVBMT (REU)
  • Filloux Denis, CIRAD-BIOS-UMR BGPI (FRA)
  • Varsani Arvind, Arizona State University (USA)
  • Roumagnac Philippe, CIRAD-BIOS-UMR BGPI (FRA) ORCID: 0000-0001-5002-6039
  • Martin Darren Patrick, UCT (ZAF)
  • Lett Jean-Michel, CIRAD-BIOS-UMR PVBMT (REU)
  • Lefeuvre Pierre, CIRAD-BIOS-UMR PVBMT (REU) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/593395/)

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