Molecular viral diagnosis and sanitation of yam genetic resources: Implications for safe yam germplasm exchange

Umber Marie, Filloux Denis, Gélabale Suzia, Gomez Rose-Marie, Marais Armelle, Gallet Séverine, Gamiette Franciane, Pavis Claudie, Teycheney Pierre-Yves. 2020. Molecular viral diagnosis and sanitation of yam genetic resources: Implications for safe yam germplasm exchange. Viruses, 12 (10):1101, 18 p.

https://doi.org/10.3390/v12101101

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Quartile : Q2, Sujet : VIROLOGY

Résumé : Yam (Dioscorea spp.) is an important crop in tropical and subtropical regions. Many viruses have been recently identified in yam, hampering genetic conservation and safe international exchanges of yam germplasm. We report on the implementation of reliable and cost-effective PCR-based detection tools targeting eight different yam-infecting viruses. Viral indexing of the in vitro yam collection maintained by the Biological Resources Center for Tropical Plants (BRC-TP) in Guadeloupe (French West Indies) unveiled a high prevalence of potyviruses, badnaviruses, Dioscorea mosaic associated virus (DMaV) and yam asymptomatic virus 1 (YaV1) and a high level of coinfections. Infected yam accessions were subjected to a combination of thermotherapy and meristem culture. Sanitation levels were monitored using PCR-based and high-throughput sequencing-based diagnosis, confirming the efficacy and reliability of PCR-based detection tools. Sanitation rates were highly variable depending on viruses. Sixteen accessions were successfully sanitized, paving the way to safe yam germplasm exchanges and the implementation of clean seed production programs worldwide.

Mots-clés Agrovoc : Dioscorea, diagnostic de laboratoire, virus des végétaux, collection botanique, conservation du matériel génétique, procédures opérationnelles normalisées d'assainissement, PCR

Mots-clés géographiques Agrovoc : Guadeloupe, France

Mots-clés complémentaires : diagnostic moléculaire

Mots-clés libres : Molecular diagnosis, Yam, Viruses, Sanitation, Germplasm conservation and dissemination

Classification Agris : H20 - Maladies des plantes

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

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