Biochemical and molecular characterization and expression of the 11S-type storage protein from Coffea arabica endosperm

Rogers William John, Bezard Vincent, Deshayes Alain, Meyer Inge, Pétiard Vincent, Marraccini Pierre. 1999. Biochemical and molecular characterization and expression of the 11S-type storage protein from Coffea arabica endosperm. Plant Physiology and Biochemistry, 37 (4) : 261-272.

https://doi.org/10.1016/S0981-9428(99)80024-2

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Résumé : In order to define better the endosperm protein content of commercial coffee species Coffea arabica (Arabica) and C. canephora (Robusta), the principal storage protein of coffee grains has been analysed by 2-dimensional electrophoresis (2DE) and amino acid microsequencing. The most abundant polypeptide spots observed on mature coffee grain 2DE profiles were found to be subunits of the same protein, which exists as multiple isoforms with varying pIs. Strong sequence similarity was found to the 11S family of plant storage proteins. The structure is typical of the 11S type, which occurs as a precursor of 55 kDa, and is observed under denaturing and reducing conditions on 2DE profiles in the form of cleavage products at approximately 20 kDa (β arms) and 32 kDa (α arms). Differences between Arabica and Robusta 2DE profiles indicate a secondary 11S protein family in some varieties of the latter. The existence of multiple pI forms may indicate that a multigene family encodes for these proteins. We estimate that the protein accounts for approximately 45 % of total grain protein. A cloned full-length cDNA of 1 706 bp coding for one of the isoforms is described and discussed in relation to other coffee storage protein sequences.

Mots-clés Agrovoc : expression des gènes, phytogénétique, Coffea arabica, Coffea canephora, endosperme, protéine de réserve des graines, biochimie, électrophorèse, séquence nucléotidique

Mots-clés complémentaires : électrophorèse bidimensionnelle

Mots-clés libres : Coffee, Gene expression, Bean development

Classification Agris : F30 - Génétique et amélioration des plantes
F60 - Physiologie et biochimie végétale

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