Occurrence of two races of Puccinia kuehnii causing orange rust of sugarcane in Florida

Sanjel Santosh, Hincapie Martha, Wang Yunwen, Todd James, Chaulagain Bhim, Sood Sushma, Comstock Jack C., Raid Richard Neil, Rott Philippe. 2021. Occurrence of two races of Puccinia kuehnii causing orange rust of sugarcane in Florida. Plant Pathology, 70 (7) : 1616-1625.

Quartile : Q2, Sujet : AGRONOMY / Quartile : Q2, Sujet : PLANT SCIENCES

Résumé : Orange rust of sugarcane caused by Puccinia kuehnii was first reported in Florida in 2007. Since then, several sugarcane cultivars that were resistant during the initial epidemics became susceptible within a few years. These shifts in resistance were attributed to the evolution of the pathogen and appearance of new races. To study the variation in virulence of P. kuehnii, healthy leaf pieces of sugarcane cultivars susceptible to orange rust were brush inoculated with isolates of P. kuehnii collected from susceptible cultivars in the field. After inoculation, leaf pieces were placed in an incubator and disease severity based on the number of rust uredinia was determined 2 weeks postinoculation. Isolates of P. kuehnii collected from sugarcane cultivar CP 89-2143, which only showed severe symptoms of orange rust starting in 2011–2012, produced 300%–500% more uredinia on CP 89-2143 than the isolates collected from cultivar CL 85-1040 that has been susceptible since 2007. Sugarcane cultivar CL 85-1040 exhibited high and equivalent numbers of uredinia regardless of the inoculated isolate of the pathogen. These data support the occurrence of pathogenic specialization within P. kuehnii and the existence of at least two races of this pathogen in Florida. Analysis of amplified fragment-length polymorphism among isolates of P. kuehnii from cultivars CP 89-2143 and CL 85-1040 differing in resistance to orange rust revealed genetic variation among rust uredinia. However, this variation was not associated with a specific sugarcane cultivar, suggesting that pathogenic variation was not linked to major, but rather to small genetic changes within the genome of P. kuehnii.

Mots-clés Agrovoc : Saccharum, agent pathogène, Pucciniales, variété, variation génétique, pouvoir pathogène

Mots-clés géographiques Agrovoc : Floride, États-Unis d'Amérique

Mots-clés complémentaires : rouille orangée, Puccinia kuchnii

Mots-clés libres : AFLP, Detached leaf assay, Saccharum spp.

Classification Agris : H20 - Maladies des plantes

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Auteurs et affiliations

• Sanjel Santosh, University of Florida (USA)
• Hincapie Martha, University of Florida (USA)
• Wang Yunwen, University of Florida (USA)
• Todd James, USDA (USA)
• Chaulagain Bhim, University of Florida (USA)
• Sood Sushma, USDA (USA)
• Comstock Jack C., USDA (USA)
• Raid Richard Neil, University of Florida (USA)
• Rott Philippe, CIRAD-BIOS-UMR PHIM (FRA) ORCID: 0000-0001-6085-6159 - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/598424/)

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