Fungal glutathione transferases as tools to explore the chemical diversity of amazonian wood extractives

Perrot Thomas, Schwartz Mathieu, Saiag Fanny, Salzet Guillaume, Dumarçay Stéphane, Favier Frédérique, Gérardin Philippe, Girardet Jean-Michel, Sormani Rodnay, Morel-Rouhier Mélanie, Amusant Nadine, Didierjean Claude, Gelhaye Eric. 2018. Fungal glutathione transferases as tools to explore the chemical diversity of amazonian wood extractives. ACS Sustainable Chemistry and Engineering, 6 (10) : 13078-13085.

https://doi.org/10.1021/acssuschemeng.8b02636

Article de revue ; Article de recherche ; Article de revue à facteur d'impact

Version publiée - Anglais
Accès réservé aux personnels Cirad
Utilisation soumise à autorisation de l'auteur ou du Cirad.
598821.pdf
Télécharger (2MB) | Demander une copie

Quartile : Outlier, Sujet : ENGINEERING, CHEMICAL / Quartile : Q1, Sujet : GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY (Science) / Quartile : Q1, Sujet : CHEMISTRY, MULTIDISCIPLINARY

Résumé : The natural durability of wood is linked to its chemical composition and in particular the presence of metabolites called extractives that often possess chemical reactivity. For dealing with these compounds, wood degraders have developed detoxification systems usually involving enzyme families. Among these enzymes, glutathione transferases (GSTs) are involved in the decrease of the reactivity of toxic compounds. In this study, the hypothesis that the detoxification systems of wood decaying fungi could be indicators of the chemical reactivity of wood extracts has been tested. This approach has been evaluated using 32 wood extracts coming from French Guiana species, testing their antimicrobial ability, antioxidative properties, and reactivity against six GSTs from the white rot Trametes versicolor. From the obtained data, a significant correlation between the antimicrobial and antioxidative properties of the tested wood extracts and GST interactions was established. In addition, the chemical analysis performed on one of the most reactive extracts (an acetonic extract of Bagassa guianensis) has demonstrated oxyresveratrol as a major constituent. We were able to cocrystallize one GST with this commercially interesting compound. Taken together, the presented data support the hypothesis that detoxifying enzymes could be used to identify the presence of molecules of industrial interest in wood extracts.

Mots-clés Agrovoc : technologie du bois, propriété antimicrobienne, composition chimique, extrait de bois, glutathion, propriété antioxydant

Mots-clés géographiques Agrovoc : Amazonie, Guyane française, France

Mots-clés complémentaires : Glutathion transférase, Bagassa guianensis, Trametes versicolor

Mots-clés libres : Trametes versicolor, Amazonian wood species, Bagassa guianensis, Glutathione transferases, Stilbene

Classification Agris : K50 - Technologie des produits forestiers
U50 - Sciences physiques et chimie

Champ stratégique Cirad : Hors axes (2014-2018)

Auteurs et affiliations