Ebb and flow of vascular pathogenesis through repeated gain

Gluck-Thaler E., Cerutti Aude, Perez Quintero A.L., Pesce C., Jauneau Alain, Vancheva T., Lang Jillian M., Allen Caitilyn, Verdier-Michel V., Gagnevin Lionel, Szurek Boris, Cunnac Sébastien, Bragard Claude, Leach Jan E., Noel Laurent D., Slot J., Koebnik Ralf, Jacobs J.M.. 2019. Ebb and flow of vascular pathogenesis through repeated gain. Molecular Plant-Microbe Interactions, 32 (10S), suppl., p. S1.22 IS-MPMI Congress. 18, Glasgow, Royaume-Uni, 14 Juillet 2019/18 Juillet 2019.

Quartile : Q1, Sujet : PLANT SCIENCES / Quartile : Q2, Sujet : BIOTECHNOLOGY & APPLIED MICROBIOLOGY / Quartile : Q2, Sujet : BIOCHEMISTRY & MOLECULAR BIOLOGY

Note générale : Le volume 32, numéro 10S de Molecular Plant-Microbe Interactions est consacré à l'IS-MPMI XVIII Congress : Abstracts of Presentations at IS-MPMI XVIII Congress, pages S1.1–S1.212

Résumé : Microbes cause disease in plants through two distinct processes. Vascular pathogens move through host veins leading to widespread infection, while non-vascular pathogens remain restricted to the site of infection. The systemic nature of vascular pathogenicity poses a much greater risk to host health; yet the mechanisms underpinning this lifestyle are unknown. Here, we examine the molecular and evolutionary basis for vascular athogenicity in Xanthomonas, a diverse genus of plant-associated bacteria that cause vascular and non-vascular diseases. As opposed to equally complex traits controlled by multiple loci, we identified a single gene, celA (encoding a cellobiosidase), that acts as a switch for vascular pathogenesis. Heterologous expression of celA in non-vascular Xanthomonas species resulted in pathotype conversion allowing for xylem colonization, while its deletion in vascular species resulted in the loss of xylem colonization, demonstrating that celA is both sufficient and necessary for vascular pathogenesis. We inferred several gains and losses of celA in Xanthomonas, where celA was acquired through horizontal transfer in what are now vascular lineages, and alternatively lost through transposon-mediated insertion in non-vascular lineages. The dynamic evolution of celA suggests that rather than representing evolutionary endpoints, vascular and non-vascular modes of infection exist on a continuum, and populations can easily flow from one end to another.

Mots-clés Agrovoc : Xanthomonas, maladie des plantes, xylème, tissu vasculaire, maladie vasculaire, gène, expression des gènes

Classification Agris : H20 - Maladies des plantes

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Auteurs et affiliations

• Gluck-Thaler E., Ohio State University (USA) - auteur correspondant
• Cerutti Aude, INRA (FRA)
• Perez Quintero A.L., Colorado State University (USA)
• Pesce C., University of New Hampshire (USA)
• Jauneau Alain, CNRS (FRA)
• Vancheva T., UCL (BEL)
• Lang Jillian M., Colorado State University (USA)
• Allen Caitilyn, University of Wisconsin (USA)
• Verdier-Michel V., IRD (FRA)
• Gagnevin Lionel, CIRAD-BIOS-UMR IPME (FRA)
• Szurek Boris, IRD (FRA)
• Cunnac Sébastien, IRD (FRA)
• Bragard Claude, UCL (BEL)
• Leach Jan E., Colorado State University (USA)
• Noel Laurent D., INRA (FRA)
• Slot J., Ohio State University (USA)
• Koebnik Ralf, IRD (FRA)
• Jacobs J.M., Ohio State University (USA)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/598926/)

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