Structure and sequence of the sex determining locus in two wild populations of Nile tilapia

Triay Cécile, Conte Matthew A., Baroiller Jean-François, Bezault Etienne, Clark Frances E., Penman David J., Kocher Thomas D., D'Cotta Helena. 2020. Structure and sequence of the sex determining locus in two wild populations of Nile tilapia. Genes, 11 (9):1017, 28 p.

Quartile : Q2, Sujet : GENETICS & HEREDITY

Résumé : In domesticated strains of the Nile tilapia, phenotypic sex has been linked to genetic variants on linkage groups 1, 20 and 23. This diversity of sex-loci might reflect a naturally polymorphic sex determination system in Nile tilapia, or it might be an artefact arising from the process of domestication. Here, we searched for sex-determiners in wild populations from Kpandu, Lake Volta (Ghana-West Africa), and from Lake Koka (Ethiopia-East Africa) that have not been subjected to any genetic manipulation. We analysed lab-reared families using double-digest Restriction Associated DNA sequencing (ddRAD) and analysed wild-caught males and females with pooled whole-genome sequencing (WGS). Strong sex-linked signals were found on LG23 in both populations, and sex-linked signals with LG3 were observed in Kpandu samples. WGS uncovered blocks of high sequence coverage, suggesting the presence of B chromosomes. We confirmed the existence of a tandem amh duplication in LG23 in both populations and determined its breakpoints between the oaz1 and dot1l genes. We found two common deletions of ~5 kb in males and confirmed the presence of both amhY and amh∆Y genes. Males from Lake Koka lack both the previously reported 234 bp deletion and the 5 bp frameshift-insertion that creates a premature stop codon in amh∆Y.

Mots-clés Agrovoc : génétique animale, détermination du sexe, sexage, Oreochromis niloticus, locus, structure génétique

Mots-clés géographiques Agrovoc : Ghana, Éthiopie

Mots-clés libres : Oreochromis niloticus, Nile tilapia, Sex chromosomes, Sex determining locus, Sex determination

Classification Agris : L10 - Génétique et amélioration des animaux

Champ stratégique Cirad : CTS 1 (2019-) - Biodiversité

Agences de financement hors UE : Agence Nationale de la Recherche

Auteurs et affiliations

• Triay Cécile, CIRAD-PERSYST-UMR ISEM (FRA)
• Conte Matthew A., University of Maryland (USA)
• Baroiller Jean-François, CIRAD-PERSYST-UMR ISEM (FRA) ORCID: 0000-0002-0992-1725
• Bezault Etienne, Université des Antilles (GLP)
• Clark Frances E., University of Maryland (USA)
• Penman David J., University of Stirling (GBR)
• Kocher Thomas D., University of Maryland (USA)
• D'Cotta Helena, CIRAD-PERSYST-UMR ISEM (FRA) ORCID: 0000-0003-2739-3476 - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/599558/)

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