Molecular detection and quantification of Xanthomonas albilineans in juice from symptomless sugarcane stalks using a real-time quantitative PCR assay

Shi Yang, Zhao Jian-Ying, Zhou Jing-Ru, Ntambo Mbuya Sylvain, Xu Peng-Yuan, Rott Philippe, Cao San-Ji. 2021. Molecular detection and quantification of Xanthomonas albilineans in juice from symptomless sugarcane stalks using a real-time quantitative PCR assay. Plant Disease, 105 (11) : 3451-3458.

Quartile : Q1, Sujet : PLANT SCIENCES

Résumé : Leaf scald, a bacterial disease caused by Xanthomonas albilineans (Ashby) Dowson, is a major limiting factor for sugarcane production worldwide. Accurate identification and quantification of X. albilineans is a prerequisite for successful management of this disease. A sensitive and robust quantitative PCR (qPCR) assay was developed in this study for detection and quantification of X. albilineans using TaqMan probe and primers targeting a putative adenosine triphosphate–binding cassette (ABC) transporter gene (abc). The novel qPCR assay was highly specific to the 43 tested X. albilineans strains belonging to different pulsed-field gel electrophoresis groups. The detection thresholds were 100 copies/µl of plasmid DNA, 100 fg/µl of bacterial genomic DNA, and 100 CFU/ml of bacterial suspension prepared from pure culture. This qPCR assay was 100 times more sensitive than a conventional PCR assay. The pathogen was detected by qPCR in 75.1% (410/546) of symptomless stalk samples, whereas only 28.4% (155/546) of samples tested positive by conventional PCR. Based on qPCR data, population densities of X. albilineans in symptomless stalks of the same varieties differed between two sugarcane production areas in China, Beihai (Guangxi Province) and Zhanjiang (Guangdong Province), and no significant correlation between these populations was identified. Furthermore, no relationship was found between these populations of the pathogen in asymptomatic stalks and the resistance level of the sugarcane varieties to leaf scald. The newly developed qPCR assay proved to be highly sensitive and reliable for the detection and quantification of X. albilineans in sugarcane stalks.

Mots-clés Agrovoc : Xanthomonas albilineans, PCR, Saccharum officinarum, identification, Saccharum, maladie bactérienne, technique analytique, Xanthomonas, maladie des plantes, adn, diagnostic

Mots-clés géographiques Agrovoc : France, Chine, Guadeloupe

Mots-clés libres : Leaf scald, Molecular detection, Real-time quantitative PCR, Saccharum spp., Xanthomonas albilineans

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Auteurs et affiliations

• Shi Yang, Fujian Agriculture and Forestry University (CHN)
• Zhao Jian-Ying, Fujian Agriculture and Forestry University (CHN)
• Zhou Jing-Ru, Fujian Agriculture and Forestry University (CHN)
• Ntambo Mbuya Sylvain, UNILU (COD)
• Xu Peng-Yuan, Fujian Agriculture and Forestry University (CHN)
• Rott Philippe, CIRAD-BIOS-UMR PHIM (FRA) ORCID: 0000-0001-6085-6159 - auteur correspondant
• Cao San-Ji, Fujian Agriculture and Forestry University (CHN) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/600040/)

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