Potato-infecting Ralstonia solanacearum strains in Iran expand knowledge on the global diversity of brown rot ecotype of the pathogen

Sedighian Nasim, Taghavi S. Mohsen, Hamzehzarghani Habiballah, van der Wolf Jan M., Wicker Emmanuel, Osdaghi Ebrahim. 2020. Potato-infecting Ralstonia solanacearum strains in Iran expand knowledge on the global diversity of brown rot ecotype of the pathogen. Phytopathology, 110 (10) : 1647-1656.

https://doi.org/10.1094/PHYTO-03-20-0072-R

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Quartile : Q1, Sujet : PLANT SCIENCES

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Psychologie-éthologie-ergonomie

Résumé : Bacterial wilt and brown rot disease caused by Ralstonia solanacearum species complex (RSSC) is one of the major constraints of potato (Solanum tuberosum) production around the globe. During 2017 to 2018, an extensive field survey was conducted in six potato-growing provinces of Iran to monitor the status of bacterial wilt disease. Pathogenicity and host range assays using 59 bacterial strains isolated in Iran showed that they were pathogenic on eggplant, red nightshade, pepper, potato and tomato, while nonpathogenic on common bean, cowpea, cucumber, sunflower, zinnia and zucchini. PCR-based diagnosis revealed that the strains belong to the phylotype IIB/sequevar 1 (IIB/I) lineage of the RSSC. Furthermore, a five-gene multilocus sequence analysis and typing (egl, fliC, gyrB, mutS, and rplB) confirmed the phylogenetically near-homogeneous nature of the strains within IIB/I lineage. Four sequence types were identified among 58 IIB/1 strains isolated in Iran. Phylogenetically near-homogeneous nature of the strains in Iran raise questions about the mode of inoculum entry of the bacterial wilt pathogen into the country (one-time introduction versus multiple introductions), while the geographic origin of the Iranian R. solanacearum strains remains undetermined. Furthermore, sequence typing showed that there were shared alleles (haplotypes) and sequence types among the strains isolated in geographically distant areas in Iran, suggesting intranational transmission of the pathogen in the country.

Mots-clés Agrovoc : pathologie végétale, Enquête pathologique, flétrissement, maladie bactérienne, Ralstonia solanacearum, Solanum tuberosum

Mots-clés géographiques Agrovoc : Iran (République islamique d')

Mots-clés complémentaires : Flétrissement bactérien, Pourriture brune

Mots-clés libres : Bacterial wilt, Phylotype, Ralstonia solanacearum species complex, Solanaceous crops, Solanum tuberosum

Classification Agris : H20 - Maladies des plantes

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

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