Identification of QTLs controlling resistance to anthracnose disease in water yam (Dioscorea alata)

Agre Paterne Angelot, Darkwa Kwabena, Olasanmi Bunmi, Kolade Olufisayo, Mournet Pierre, Bhattacharjee Ranjana, Lopez-Montes Antonio, De Koeyer David, Adebola Patrick, Kumar Lava, Asiedu Robert, Asfaw Asrat. 2022. Identification of QTLs controlling resistance to anthracnose disease in water yam (Dioscorea alata). Genes, 13 (2), n.spéc. Research on Tropical Food Crop Genomics:347, 16 p.

Résumé : Anthracnose disease caused by a fungus Colletotrichum gloeosporioides is the primary cause of yield loss in water yam (Dioscorea alata), the widely cultivated species of yam. Resistance to yam anthracnose disease (YAD) is a prime target in breeding initiatives to develop durable-resistant cultivars for sustainable management of the disease in water yam cultivation. This study aimed at tagging quantitative trait loci (QTL) for anthracnose disease resistance in a bi-parental mapping population of D. alata. Parent genotypes and their recombinant progenies were genotyped using the Genotyping by Sequencing (GBS) platform and phenotyped in two crop cycles for two years. A high-density genetic linkage map was built with 3184 polymorphic Single Nucleotide Polymorphism (NSP) markers well distributed across the genome, covering 1460.94 cM total length. On average, 163 SNP markers were mapped per chromosome with 0.58 genetic distances between SNPs. Four QTL regions related to yam anthracnose disease resistance were identified on three chromosomes. The proportion of phenotypic variance explained by these QTLs ranged from 29.54 to 39.40%. The QTL regions identified showed genes that code for known plant defense responses such as GDSL-like Lipase/Acylhydrolase, Protein kinase domain, and F-box protein. The results from the present study provide valuable insight into the genetic architecture of anthracnose resistance in water yam. The candidate markers identified herewith form a relevant resource to apply marker-assisted selection as an alternative to a conventional labor-intensive screening for anthracnose resistance in water yam.

Mots-clés Agrovoc : anthracnose, locus des caractères quantitatifs, Dioscorea alata, résistance aux maladies, polymorphisme à nucléotide unique, génotype, génome, phénotype

Mots-clés géographiques Agrovoc : Nigéria

Mots-clés complémentaires : Colletotrichum gloeosporioides

Mots-clés libres : Dioscorea spp., Greater yam, Genetic map, Marker–trait association, Linkage analysis

Classification Agris : H20 - Maladies des plantes
F30 - Génétique et amélioration des plantes

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Auteurs et affiliations

• Agre Paterne Angelot, IITA (NGA)
• Darkwa Kwabena, SARI (GHA)
• Olasanmi Bunmi, University of Ibadan (NGA)
• Kolade Olufisayo, IITA (NGA)
• Mournet Pierre, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0001-8011-8647
• Bhattacharjee Ranjana, IITA (NGA)
• Lopez-Montes Antonio, IITA (NGA)
• De Koeyer David, Agriculture and Agri-Food Canada (CAN)
• Adebola Patrick, IITA (NGA)
• Kumar Lava, IITA (NGA)
• Asiedu Robert, IITA (NGA)
• Asfaw Asrat, International Livestock Research Institute (ETH) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/600297/)

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