Genomic approach to study floral development genes in Rosa sp.

Dubois Annick, Remay Arnaud, Raymond Olivier, Balzergue Sandrine, Chauvet Aurélie, Maene Marion, Pecrix Yann, Yang Shu-Hua, Jeauffre julien, Thouroude Tatiana, Boltz Véronique, Martin-Magniette Marie-Laure, Janczarski Stéphane, Legeai Fabrice, Renou Jean-Pierre, Vergne Philippe, Le Bris Manuel, Foucher Fabrice, Bendahmane Mohammed. 2011. Genomic approach to study floral development genes in Rosa sp.. PloS One, 6 (12):e28455, 14 p.

Quartile : Q1, Sujet : BIOLOGY

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Psychologie-éthologie-ergonomie; Staps

Résumé : Cultivated for centuries, the varieties of rose have been selected based on a number of flower traits. Understanding the genetic and molecular basis that contributes to these traits will impact on future improvements for this economically important ornamental plant. In this study, we used scanning electron microscopy and sections of meristems and flowers to establish a precise morphological calendar from early rose flower development stages to senescing flowers. Global gene expression was investigated from floral meristem initiation up to flower senescence in three rose genotypes exhibiting contrasted floral traits including continuous versus once flowering and simple versus double flower architecture, using a newly developed Affymetrix microarray (Rosa1_Affyarray) tool containing sequences representing 4765 unigenes expressed during flower development. Data analyses permitted the identification of genes associated with floral transition, floral organs initiation up to flower senescence. Quantitative real time PCR analyses validated the mRNA accumulation changes observed in microarray hybridizations for a selection of 24 genes expressed at either high or low levels. Our data describe the early flower development stages in Rosa sp, the production of a rose microarray and demonstrate its usefulness and reliability to study gene expression during extensive development phases, from the vegetative meristem to the senescent flower.

Mots-clés Agrovoc : phytogénétique, génomique, expression des gènes, stade de développement végétal, floraison, méristème, Rosa

Mots-clés libres : Roses, Flowers, Gene expression, Floral development, Microarrays, Transcription factors, Meristems, Membrane proteins

Classification Agris : F30 - Génétique et amélioration des plantes
F62 - Physiologie végétale - Croissance et développement

Champ stratégique Cirad : Axe 6 (2005-2013) - Agriculture, environnement, nature et sociétés

Auteurs et affiliations

• Dubois Annick, CNRS (FRA)
• Remay Arnaud, INRA (FRA)
• Raymond Olivier, CNRS (FRA)
• Balzergue Sandrine, INRA (FRA)
• Chauvet Aurélie, INRA (FRA)
• Maene Marion, INRA (FRA)
• Pecrix Yann, Institut méditerranéen d'écologie et de paléoécologie (FRA) ORCID: 0000-0002-6537-3145
• Yang Shu-Hua, INRA (FRA)
• Jeauffre julien, INRA (FRA)
• Thouroude Tatiana, INRA (FRA)
• Boltz Véronique, INRA (FRA)
• Martin-Magniette Marie-Laure, CNRS (FRA)
• Janczarski Stéphane, INRA (FRA)
• Legeai Fabrice, INRA (FRA)
• Renou Jean-Pierre, INRA (FRA)
• Vergne Philippe, INRA (FRA)
• Le Bris Manuel, Institut méditerranéen d'écologie et de paléoécologie (FRA)
• Foucher Fabrice, INRA (FRA)
• Bendahmane Mohammed, INRA (FRA) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/600453/)

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