Identification and validation of QTLs for tuber quality related traits in greater yam (Dioscorea alata L.)

Ehounou Adou Emmanuel, Cormier Fabien, Maledon Erick, Nudol Elie, Vignes Hélène, Gravillon Marie-Claire, N'Guetta Assanvo Simon-Pierre, Mournet Pierre, Chaïr Hâna, Kouakou Amani Michel, Arnau Gemma. 2022. Identification and validation of QTLs for tuber quality related traits in greater yam (Dioscorea alata L.). Scientific Reports, 12:8423, 14 p.

Url - jeu de données - Entrepôt autre : https://www.ncbi.nlm.nih.gov/bioproject/PRJNA515897 / Url - jeu de données - Entrepôt autre : https://yambase.org/

Résumé : Two Dioscorea alata populations were generated by hand pollination between contrasted diploid genitors. Population A (74F × Kabusa) was composed of 121 progenies while population B (74F × 14M) involved 193 progenies. These two populations were assessed over two consecutive years regarding important tuber quality traits. Analysis of variance showed that the genotype had the greatest influence on the phenotypic scores. Also for some traits, effect of the year_replicate was strong. The heritabilities of most traits were high. Based on these data and a reference high-density genetic map of greater yam, a total of 34 quantitative trait loci (QTLs) were detected on 8 of the 20 yam chromosomes. They corresponded to five of each of the following traits: tuber size, shape regularity, tubercular roots, skin texture, tuber flesh oxidation, six for oxidation ratio and three for flesh colour. The fraction of total phenotypic variance attributable to a single QTL ranged from 11.1 to 43.5%. We detected significant correlations between traits and QTL colocalizations that were consistent with these correlations. A majority of QTLs (62%) were found on linkage group LG16, indicating that this chromosome could play a major role in genetic control of the investigated traits. In addition, an inversion involving this chromosome was detected in the Kabusa male. Nine QTLs were validated on a diversity panel, including three for tuber size, three for shape regularity, two for skin texture and one for tubercular roots. The approximate physical localization of validated QTLs allowed the identification of various candidates genes. The validated QTLs should be useful for breeding programs using marker-assisted selection to improve yam tuber quality.

Mots-clés Agrovoc : phytogénétique, héritabilité, locus des caractères quantitatifs, génotype, amélioration des plantes, tubercule, biotechnologie végétale, qualité des aliments, Dioscorea alata

Mots-clés géographiques Agrovoc : Guadeloupe, France

Mots-clés libres : Dioscorea alata, Tuber quality, Quantitative trait loci, QTL validation, Chomosomal inversion

Classification Agris : F30 - Génétique et amélioration des plantes
Q04 - Composition des produits alimentaires

Champ stratégique Cirad : CTS 2 (2019-) - Transitions agroécologiques

Agences de financement hors UE : Bill and Melinda Gates Foundation, Centre de Coopération Internationale en Recherche Agronomique pour le Développement

Projets sur financement : (FRA) Enhancing Yam Breeding for Increased Productivity and Improved Quality in West Africa

Auteurs et affiliations

• Ehounou Adou Emmanuel, CIRAD-BIOS-UMR AGAP (GLP)
• Cormier Fabien, Université de Montpellier (FRA) ORCID: 0000-0002-7283-4616
• Maledon Erick, CIRAD-BIOS-UMR AGAP (GLP)
• Nudol Elie, CIRAD-BIOS-UMR AGAP (GLP)
• Vignes Hélène, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0002-8725-2467
• Gravillon Marie-Claire, CIRAD-BIOS-UMR AGAP (GLP)
• N'Guetta Assanvo Simon-Pierre, UFHB (CIV)
• Mournet Pierre, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0001-8011-8647
• Chaïr Hâna, CIRAD-BIOS-UMR AGAP (FRA)
• Kouakou Amani Michel, CNRA (CIV)
• Arnau Gemma, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0003-0714-6830 - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/601025/)

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