Differential gene expression in Eucalyptus clones in response to nutrient deficiency

Rossini Bruno Cesar, Bouvet Jean-Marc, Frouin Julien, Guerrini Iraê Amaral, Moreira de Freitas Talitha Casella, Ribeiro da Silva Magali, Bouillet Jean-Pierre, Laclau Jean-Paul, Marino Celso Luis. 2022. Differential gene expression in Eucalyptus clones in response to nutrient deficiency. Tree Genetics and Genomes, 18:14, 17 p.

https://doi.org/10.1007/s11295-022-01546-x

Article de revue ; Article de recherche ; Article de revue à facteur d'impact

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Rossini 2022_TGG_Differential gene expression in Eucalyptus clones in response to nutrient deficiency.pdf
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Url - jeu de données - Entrepôt autre : https://www.ncbi.nlm.nih.gov/biosample/?term=SAMN14669553

Résumé : We evaluated differential expression of genes in leaf and xylem tissues for three Eucalyptus clones in the field using Illumina sequencing, under four contrasting fertilization regimes: a control combining nitrogen (N), phosphorus (P), and potassium (K) and three regimes with N, K, and P deficiency. The field results showed significantly better performance with a control fertilizing regime for height and circumference at 14 months, but no differences between clones. The number of up and down regulated DEG (differentially expressed genes) in pairwise clone comparisons was around 5900 for leaf and 6900 for xylem at FDR < 0.01. With fertilization treatment comparisons, DEG were only observed for N deficiency versus control with 45 up and down-regulated DEG for leaf and 1022 for xylem. The number of DEGs between fertilizer deficiency treatment and control varied greatly within each clone showing important clone by fertilization interaction. Gene ontology analysis showed that a great number of genes were related to stress, transport, and transcription factors. The co-expression analysis showed some significant correlations between complex network gene expression and tree growth induced by fertilization regime. For example, the co-expression of a 54 DEG network showed a significant correlation with tree height (0.84, P value: 1e−04) and DBH (0.89, P value: 7e−06) in the case of N deficiency versus control. Our results suggest that gene expression levels between different fertilization treatments and clones can provide a basis for future research on gene function in Eucalyptus under nutrient stress with the perspective of new development in Eucalyptus breeding.

Mots-clés Agrovoc : carence en substance nutritive, expression des gènes, Eucalyptus, clone, phytogénétique, réponse de la plante

Mots-clés libres : Transcriptome, Fertilizer, Co-expression network, Height, DBH, Correlation

Classification Agris : F30 - Génétique et amélioration des plantes
F61 - Physiologie végétale - Nutrition

Champ stratégique Cirad : CTS 2 (2019-) - Transitions agroécologiques

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