NIRS acquisition on fresh matooke fingers using the benchtop NIRS FOSS DS2500 and relating spectra to finger dry matter content by oven method. High-throughput phenotyping protocols (HTPP), WP3

Nuwamanya Ephraim, Alamu Emmanuel, Adesokan Michael, Meghar Karima, Davrieux Fabrice. 2021. NIRS acquisition on fresh matooke fingers using the benchtop NIRS FOSS DS2500 and relating spectra to finger dry matter content by oven method. High-throughput phenotyping protocols (HTPP), WP3. Kampala : RTBfoods Project-CIRAD, 11 p.

Url - jeu de données - Dataverse Cirad : https://doi.org/10.18167/DVN1/OQ13EV

Résumé : Determination of dry matter is important in selection of banana with acceptable qualities for consumer utilization. DM determination in banana is quickly and reliably determined using NIRS technology. The Standard Operating Procedures (SOPs) detailed allows for the acquisition of mashed banana fingers. The procedures describe the scanning and spectral acquisition of banana using the small sample cup of the FOSS DS2500 NIRS equipment. Banana samples are harvested using approved harvesting tools and labelled after which preparation commences by peeling and grating using a grater. This is followed loading the grates into a sample cup and scanning of the grated material filled in the sample cup. The procedure is repeated by loading fresh material from the main sample into the sample cup producing two subsets of scans from one particular accession. Spectral data produced from these scans is downloaded and further processed for use in calibration development and finger/cluster chemical composition determination. Reference data generation is carried out by approved reference methods in repeatability analyses carried out at NaCRRI and NARL. Critical points of consideration include the development of a sample flow and spectra acquisition matrix coupled to correct labelling since sample numbers involved are usually many.

Mots-clés libres : Banana, Spectra, Procedure, Reference data, Repeatability

Agences de financement hors UE : Bill and Melinda Gates Foundation, Centre de Coopération Internationale en Recherche Agronomique pour le Développement, Centro Internacional de Agricultura Tropical, Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement, James Hutton Institute

Projets sur financement : (FRA) Breeding RTB Products for End User Preferences

Auteurs et affiliations

• Nuwamanya Ephraim, NaCRRI (UGA)
• Alamu Emmanuel, IITA (NGA)

Contributeurs et affiliations

• Adesokan Michael, IITA (NGA) - collaborateur
• Meghar Karima, CIRAD-PERSYST-UMR Qualisud (FRA) - collaborateur
• Davrieux Fabrice, CIRAD-PERSYST-UMR Qualisud (REU) - collaborateur

Source : Cirad-Agritrop (https://agritrop.cirad.fr/602138/)

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