Assessing geminiviruses prevalence in Mediterranean vegetable crops

Belabess Zineb, Mnari Hattab Monia, Anfoka Ghandi, Granier Martine, Accotto Gian Paolo, Ballardini Massimiliano, Bernardo Umberto, Davino Salvatore, Ennmily Meryem, Jerbi Nihed, Laarif Asma, Marian Daniele, Matic Slavica, Mazza Giuseppe, Miozzi Laura, Nazih Abderrahmane, Noris Emanuela, Nugnes Francesco, Taarabt Youness, Tahiri Abdessalem, Urbino Cica, Zaagueri Takoua, Peterschmitt Michel, Vaira Anna Maria. 2022. Assessing geminiviruses prevalence in Mediterranean vegetable crops. . SFV. Sète : SFV, Résumé, 1 p. International Symposium on ssDNA Viruses, Sète, France, 26 Septembre 2022/1 Octobre 2022.

Résumé : One of the most harmful viruses to vegetable crops, especially in the Mediterranean Basin, is the family Geminiviridae known as the "geminivirus." A project on the prevention and control of new and invasive geminiviruses infecting vegetables in the Mediterranean was launched through an international 3-year collaboration federated by a PRIMA call (Partnership for research and innovation in the Mediterranean area). This project involved public and private institutions from Italy, Jordan, Tunisia, Morocco, and France. Virusspecific PCR tests on DNA extracted from symptomatic tomato and cucurbit plants have been used to monitor the prevalence of geminiviruses previously described in this region, including tomato yellow leaf curl associated viruses, tomato leaf curl New Delhi virus, chickpea chlorotic dwarf virus, squash leaf curl virus, and watermelon chlorotic stunt virus. Meanwhile, deep sequencing of DNA extracted from insects collected in the surveyed areas (mainly whiteflies, aphids, and leafhoppers) is being used to look into the possibility of novel geminiviruses circulating in the region. To compare results of viral incidence between countries, standardized procedures for collecting, preserving, and analyzing leaf and insect samples have been established. In different agroecosystems in the following countries in 2020-2021, more than a thousand leaf samples and about 200 insect samples were collected: Morocco, in the Berkane area in the North-East and Souss region in the South; Tunisia, in the Center, North, and Sahel regions; Italy, in Campania and Sicily; and the Northern area of Jordan. These surveys are being used to update data on geminivirus prevalence across the Mediterranean Basin.

Mots-clés libres : Geminivirus, Recombinant virus, Vegetable crops

Auteurs et affiliations

• Belabess Zineb, INRA [Maroc] (MAR)
• Mnari Hattab Monia, INRAT (TUN)
• Anfoka Ghandi, Al-Balqa Applied University (JOR)
• Granier Martine, CIRAD-BIOS-UMR PHIM (FRA)
• Accotto Gian Paolo, ISPP (ITA)
• Ballardini Massimiliano, Esasem Spa (ITA)
• Bernardo Umberto, National Research Council (ITA)
• Davino Salvatore, Gautier semence (FRA)
• Ennmily Meryem, ENAM (MAR)
• Jerbi Nihed, INRAT (TUN)
• Laarif Asma, INRAT (TUN)
• Marian Daniele, CNR (ITA)
• Matic Slavica, CNR (ITA)
• Mazza Giuseppe, National Research Council (ITA)
• Miozzi Laura, CNR (ITA)
• Nazih Abderrahmane, INRA [Maroc] (MAR)
• Noris Emanuela, National Research Council (ITA)
• Nugnes Francesco, National Research Council (ITA)
• Taarabt Youness, INRA [Maroc] (MAR)
• Tahiri Abdessalem, ENAM (MAR)
• Urbino Cica, CIRAD-BIOS-UMR PHIM (FRA) ORCID: 0000-0002-6215-9389
• Zaagueri Takoua, INRAT (TUN)
• Peterschmitt Michel, CIRAD-BIOS-UMR PHIM (FRA) ORCID: 0000-0001-8709-9418
• Vaira Anna Maria, National Research Council (ITA)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/602857/)

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