Insertion of badnaviral DNA in the late blight resistance gene (R1a) of eggplant (Solanum melongena)

Serfraz Saad, Sharma Vikas, Maumus Florian, Aubriot Xavier, Geering Andrew D., Teycheney Pierre-Yves. 2021. Insertion of badnaviral DNA in the late blight resistance gene (R1a) of eggplant (Solanum melongena). Frontiers in Plant Science, 12:683681, 14 p.

Quartile : Q1, Sujet : PLANT SCIENCES

Résumé : Endogenous viral elements (EVEs) are widespread in plant genomes. They result from the random integration of viral sequences into host plant genomes by horizontal DNA transfer and have the potential to alter host gene expression. We performed a large-scale search for co-transcripts including caulimovirid and plant sequences in 1,678 plant and 230 algal species and characterized 50 co-transcripts in 45 distinct plant species belonging to lycophytes, ferns, gymnosperms and angiosperms. We found that insertion of badnavirus EVEs along with Ty-1 copia mobile elements occurred into a late blight resistance gene (R1) of brinjal eggplant (Solanum melongena) and wild relatives in genus Solanum and disrupted R1 orthologs. EVEs of two previously unreported badnaviruses were identified in the genome of S. melongena, whereas EVEs from an additional novel badnavirus were identified in the genome of S. aethiopicum, the cultivated scarlet eggplant. Insertion of these viruses in the ancestral lineages of the direct wild relatives of the eggplant would have occurred during the last 3 Myr, further supporting the distinctiveness of the group of the eggplant within the giant genus Solanum.

Mots-clés Agrovoc : résistance aux maladies, mildiou oidium, Solanum melongena

Mots-clés complémentaires : Badnavirus

Mots-clés libres : Endogenous viral elements, Badnavirus, Solanum melongena, R1 gene, Co-transcripts, Phylogeny

Classification Agris : H20 - Maladies des plantes
F30 - Génétique et amélioration des plantes

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Agences de financement hors UE : Agence Nationale de la Recherche

Projets sur financement : (FRA) Séquences virales endogènes: rôle dans l'évolution des virus et fonctions chez les plantes

Auteurs et affiliations

• Serfraz Saad, CIRAD-BIOS-UMR AGAP (GLP)
• Sharma Vikas, INRAE (FRA)
• Maumus Florian, INRAE (FRA)
• Aubriot Xavier, Université Paris-Saclay (FRA)
• Geering Andrew D., QAAFI (AUS)
• Teycheney Pierre-Yves, CIRAD-BIOS-UMR AGAP (GLP) ORCID: 0000-0002-9754-0745 - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/603352/)

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