Alkaline pretreatments for sorghum and miscanthus anaerobic digestion: impacts at cell wall and tissue scales

Thomas Hélène Laurence, Nolasco Helga Felix P., Carrere Hélène, Lartaud Marc, Cao Tuong-Vi, Baptiste Christelle, Verdeil Jean-Luc. 2022. Alkaline pretreatments for sorghum and miscanthus anaerobic digestion: impacts at cell wall and tissue scales. BioEnergy Research, 15 (2) : 792-809.

https://doi.org/10.1007/s12155-021-10342-9

Article de revue ; Article de recherche ; Article de revue à facteur d'impact

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Résumé : Lignocellulosic biomass is hardly degraded during anaerobic digestion. Among a large panel of pretreatments used to improve the biodegradability of this substrate, alkaline pretreatments are recognized as the most efficient to remove lignin and therefore improve the methane production of these substrates. This article uses different histological stains (FASGA, phloroglucinol, Mäule reagent, Congo red), immunolocalization, and histological quantification on pretreated internode stem tissue section in order to decipher the mechanism of alkaline pretreatment action (CaO and NaOH) in the anatomical and lignocellulosic matrix scale of Sorghum Biomass 140 hybrid and of Miscanthus × giganteus Floridulus. A significant delignification of all tissues was observed (sclerenchyma, parenchyma, and xylem) except in the epidermis and in the internal part of the perivascular sclerenchyma. The degradation of lignin under the effect of alkaline pretreatment is accompanied by a massive unmasking of cellulose and a reduction of crystalline cellulose. This induced an increase of anaerobic digestion kinetics for both biomass and of methane yield for miscanthus. Miscanthus is rich in G-type lignins located mainly at the level of the perivascular sclerenchyma of the external internode zone which was more degraded by alkalies than the S-type lignin; this may explain the improvement of miscanthus methane potential.

Mots-clés Agrovoc : traitement anaérobique, lignocellulose, biodégradation, biodégradabilité, biogaz, méthane, paroi cellulaire, Sorghum, Miscanthus

Mots-clés libres : Alkaline pretreatments, Lignocellulosic biomass, Biogas, Histochemistry, Tissue structure, Cell wall macromolecules mapping

Classification Agris : F60 - Physiologie et biochimie végétale
Q70 - Traitement des déchets agricoles

Champ stratégique Cirad : CTS 2 (2019-) - Transitions agroécologiques

Agences de financement hors UE : Agence Nationale de la Recherche

Projets sur financement : (FRA) Biomasse pour le futur

Auteurs et affiliations