NIRS acquisition on fresh cassava roots using FOSS DS2500 and ASD quality spec and relating the NIRS spectra to fresh HCN content by Picrate method. High-Throughput Phenotyping Protocols (HTPP), WP3

Kanaabi Michael, Nuwamanya Ephraim, Meghar Karima, Tran Thierry. 2023. NIRS acquisition on fresh cassava roots using FOSS DS2500 and ASD quality spec and relating the NIRS spectra to fresh HCN content by Picrate method. High-Throughput Phenotyping Protocols (HTPP), WP3. Kampala : RTBfoods Project-CIRAD, 11 p.

Résumé : All cassava varieties are known to contain some level of cyanogenic glucosides (CGs). Linamarine and lotaustrine are the commonest CGs in cassava but linamarine is the most abundant. The linamarine is readily hydrolyzed to glucose and acetone cyanohydrin in the presence of linamarase enzyme, which is also inherently produced by the plant. Acetone cyanohydrin decomposes rapidly in neutral or alkaline solution, liberating cyanide gas. Production of free HCN in cassava roots is prevented by compartmentalization of linamerase in cell walls and linamarine and lotaustraline in the vacuole. Therefore, damage/rupture of root tissues is desired for this reaction to occur. This Standard Operating Procedure (SOP) documents steps taken during determination of cassava root HCN content using the picrate method at the National Crops Resources Research Institute (NaCRRI), under the auspices of the RTBfoods project. Furthermore, the RTBfoods project seeks to develop high-throughput phenotyping protocols to provide RTB breeders with fast, accurate, and reproducible procedures for rapid phenotyping of their populations. NIRS is one such technologies that has been widely deployed in plant phenomics. In order to build NIRS prediction models, reference data must be matched with NIRS spectra. This document provides an SOP for generating HCN reference data using the picrate method and matching this data to NIRS spectra. Calibration of HCN reference data and NIRS data is the object of a separate document.

Mots-clés libres : Cassava, HCN, Picrate method, NIRS

Agences de financement hors UE : Bill and Melinda Gates Foundation, Centre de Coopération Internationale en Recherche Agronomique pour le Développement, Centro Internacional de Agricultura Tropical, Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement, James Hutton Institute

Projets sur financement : (FRA) Breeding RTB Products for End User Preferences

Auteurs et affiliations

• Kanaabi Michael, NaCRRI (UGA)
• Nuwamanya Ephraim, NaCRRI (UGA)

Contributeurs et affiliations

• Meghar Karima, CIRAD-PERSYST-UMR Qualisud (FRA) - collaborateur
• Tran Thierry, CIRAD-PERSYST-UMR Qualisud (FRA) ORCID: 0000-0002-9557-3340 - collaborateur

Source : Cirad-Agritrop (https://agritrop.cirad.fr/603873/)

Métriques

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