The mosquito microbiome includes habitat-specific but rare symbionts

Schrieke Hans, Maignien Loïs, Constancias Florentin, Trigodet Florian, Chakloute Sarah, Rakotoarivony Ignace, Marie Albane, L'Ambert Grégory, Makoundou Patrick, Pagès Nonito, Eren A. Murat, Weill Mylène, Sicard Mathieu, Reveillaud Julie. 2022. The mosquito microbiome includes habitat-specific but rare symbionts. Computational and Structural Biotechnology Journal, 20 : 410-420.

https://doi.org/10.1016/j.csbj.2021.12.019

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Url - jeu de données - Entrepôt autre : https://zenodo.org/badge/latestdoi/402375335 / Url - jeu de données - Entrepôt autre : https://www.ebi.ac.uk/ena/browser/view/PRJEB43079

Résumé : Microbial communities are known to influence mosquito lifestyles by modifying essential metabolic and behavioral processes that affect reproduction, development, immunity, digestion, egg survival, and the ability to transmit pathogens. Many studies have used 16S rRNA gene amplicons to characterize mosquito microbiota and investigate factors that influence host-microbiota dynamics. However, a relatively low taxonomic resolution due to clustering methods based on arbitrary threshold and the overall dominance of Wolbachia or Asaia symbionts obscured the investigation of rare members of mosquito microbiota in previous studies. Here, we used high resolution Shannon entropy-based oligotyping approach to analyze the microbiota of Culex pipiens, Culex quinquefasciatus and Aedes individuals from continental Southern France and overseas Guadeloupe as well as from laboratories with or without antibiotics treatment. Our experimental design that resulted in a series of mosquito samples with a gradient of Wolbachia density and relative abundance along with high-resolution analyses of amplicon sequences enabled the recovery of a robust signal from typically less accessible bacterial taxa. Our data confirm species-specific mosquito-bacteria associations with geography as a primary factor that influences bacterial community structure. But interestingly, they also reveal co-occurring symbiotic bacterial variants within single individuals for both Elizabethkingia and Erwinia genera, distinct and specific Asaia and Chryseobacterium in continental and overseas territories, and a putative rare Wolbachia variant. Overall, our study reveals the presence of previously overlooked microdiversity and multiple closely related symbiotic strains within mosquito individuals with a remarkable habitat-specificity.

Mots-clés Agrovoc : flore microbienne, écologie microbienne, Culex pipiens, Culex quinquefasciatus, Aedes, symbiote

Mots-clés géographiques Agrovoc : Guadeloupe, Languedoc-Roussillon, France

Mots-clés libres : Microbiome, Mosquitoes, Symbionts, Microdiversity, Oligotyping

Classification Agris : L72 - Organismes nuisibles des animaux

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Agences de financement européennes : European Research Council, European Regional Development Fund

Agences de financement hors UE : Région Occitanie Pyrénées-Méditerranée, Conseil Regional de la Guadeloupe

Programme de financement européen : H2020

Projets sur financement : (EU) Ecogenomics of Mosquito-Microbe Symbiosis for Novel Control Strategies of Infectious Diseases, (EU) Maladies infectieuses humaines, animales et végétales en milieu insulaire tropical : surveillance, diagnostic, contrôle et impact

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