Shared pedigree relationships and transmission of unreduced gametes in cultivated banana

Martin Guillaume, Baurens Franc-Christophe, Labadie Karine, Hervouet Catherine, Salmon Frédéric, Marius Franck, Paulo-de-la-Reberdiere Nilda, Van Den Houwe Ines, Aury Jean-Marc, D'Hont Angélique, Yahiaoui Nabila. 2023. Shared pedigree relationships and transmission of unreduced gametes in cultivated banana. Annals of Botany, 131 (7) : 1149-1161.

Résumé : Background and aims: Cultivated bananas resulted from inter-(sub-)specific hybridizations involving Musa species and subspecies (M. acuminata subspecies, M. schizocarpa, M. balbisiana) and the subsequent selection, centuries ago, of hybrids with parthenocarpic, seedless fruits. Cultivars have low fertility and are vegetatively propagated, forming groups of somaclones. Relatively few of them, mainly triploids, are grown on a large scale and characterization of their parental relationships may be useful for breeding strategies. Here, we investigate parental relationships and gamete-type contributions among diploid and polyploid banana cultivars. Methods: We used SNP genotyping data from whole genome sequencing of 178 banana individuals including 111 cultivars, 55 wild bananas and 12 synthetic F1 hybrids. We analysed the proportion of SNP sites in accordance with direct parentage with a global statistic and along chromosomes for selected individuals. Key Results: We characterised parentage relationships for seven diploid, eleven triploid and one tetraploid cultivars. Results showed that both diploid and triploid cultivars could have contributed gametes to other banana cultivars. Diploids may have contributed 1x or 2x gametes and triploids, 1x to 3x gametes. The Mchare diploid cultivars group nowadays only found in East Africa, was found as parent of 2 diploid and 8 triploid cultivars. In five of its identified triploid offspring, corresponding to main export or locally popular dessert bananas, Mchare contributed a 2x gamete with full genome restitution without recombination. Analyses of remaining haplotypes in these Mchare offspring suggested ancestral pedigree relationships between different interspecific banana cultivars. Conclusions: Current cultivated banana resulted from different pathways of formation, with implication of recombined or un-recombined unreduced gametes produced by diploid or triploid cultivars. Identification of dessert banana's parents and the type of their contributed gametes should support the design of breeding strategies.

Mots-clés Agrovoc : Musa acuminata, polyploïdie, multiplication végétative, Musa balbisiana, variété, Musa, Musa (bananes), hybride, diploïdie, hybridation, génotype, génome, triploïdie

Mots-clés libres : Musa, Mchare, Parentage, Polyploid, Unreduced gamete

Classification Agris : F30 - Génétique et amélioration des plantes

Champ stratégique Cirad : CTS 2 (2019-) - Transitions agroécologiques

Agences de financement hors UE : Agence Nationale de la Recherche, Centre de Coopération Internationale en Recherche Agronomique pour le Développement, Agropolis Fondation

Projets sur financement : (FRA) France Génomique-INBS, (FRA) Agricultural Sciences for sustainable Development

Auteurs et affiliations

• Martin Guillaume, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0002-1801-7500 - auteur correspondant
• Baurens Franc-Christophe, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0002-5219-8771
• Labadie Karine, Genoscope (FRA)
• Hervouet Catherine, CIRAD-BIOS-UMR AGAP (FRA)
• Salmon Frédéric, CIRAD-BIOS-UMR AGAP (GLP)
• Marius Franck, CIRAD-BIOS-UMR AGAP (GLP)
• Paulo-de-la-Reberdiere Nilda, Université de Montpellier (FRA)
• Van Den Houwe Ines, Bioversity International (BEL)
• Aury Jean-Marc, Institut de génomique (FRA)
• D'Hont Angélique, CIRAD-BIOS-UMR AGAP (FRA)
• Yahiaoui Nabila, CIRAD-BIOS-UMR AGAP (FRA) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/604835/)

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