Prehaustorial local resistance to coffee leaf rust in a Mexican cultivar involves expression of salicylic acid-responsive genes

Couttolenc-Brenis Edgar, Carrion Gloria, Villain Luc, Ortega-Escalona Fernando, Ramírez-Martínez Daniel, Mata-Rosas Martín, Méndez-Bravo Alfonso. 2020. Prehaustorial local resistance to coffee leaf rust in a Mexican cultivar involves expression of salicylic acid-responsive genes. PeerJ, 8:e8345, 21 p.

https://doi.org/10.7717/peerj.8345

Article de revue ; Article de recherche ; Article de revue à facteur d'impact Revue en libre accès total

Prévisualisation

Version publiée - Anglais
Sous licence

.
605363.pdf
Télécharger (15MB) | Prévisualisation

Quartile : Q2, Sujet : MULTIDISCIPLINARY SCIENCES

Résumé : Background: In Mexico, coffee leaf rust (CLR) is the main disease that affects the Arabica coffee crop. In this study, the local response of two Mexican cultivars of Coffea arabica (Oro Azteca and Garnica) in the early stages of Hemileia vastatrix infection was evaluated. Methods: We quantified the development of fungal structures in locally-infected leaf disks from both cultivars, using qRT-PCR to measure the relative expression of two pathogenesis recognition genes (CaNDR1 and CaNBS-LRR) and three genes associated with the salicylic acid (SA)-related pathway (CaNPR1, CaPR1, and CaPR5). Results: Resistance of the cv. Oro Azteca was significantly higher than that of the cv. Garnica, with 8.2% and 53.3% haustorial detection, respectively. In addition, the non-race specific disease resistance gene (CaNDR1), a key gene for the pathogen recognition, as well as the genes associated with SA, CaNPR1, CaPR1, and CaPR5, presented an increased expression in response to infection by H. vastatrix in cv. Oro Azteca if comparing with cv. Garnica. Our results suggest that Oro Azteca's defense mechanisms could involve early recognition of CLR by NDR1 and the subsequent activation of the SA signaling pathway.

Mots-clés Agrovoc : Coffea arabica, Hemileia vastatrix, expression des gènes, maladie des plantes, rouille, résistance aux maladies, Coffea canephora, variété, Azteca, mécanisme de défense, réaction de défense des plantes, Coffea, gène