Interactions of REF1 and SRPP1 rubber particle proteins from Hevea brasiliensis with synthetic phospholipids : Effect of charge and size of lipid headgroup

Wadeesirisak Kanthida, Castano Sabine, Vaysse Laurent, Bonfils Frédéric, Peruch Frédéric, Rattanaporn Kittipong, Liengprayoon Siriluck, Lecomte Sophie, Bottier Céline. 2023. Interactions of REF1 and SRPP1 rubber particle proteins from Hevea brasiliensis with synthetic phospholipids : Effect of charge and size of lipid headgroup. Biochemical and Biophysical Research Communications, 679 : 205-214.

Résumé : According to the fatty acid and headgroup compositions of the phospholipids (PL) from Hevea brasiliensis latex, three synthetic PL were selected (i.e. POPA: 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphate POPC: 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine and POPG: 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol) to investigate the effect of PL headgroup on the interactions with two major proteins of Hevea latex, i.e. Rubber Elongation Factor (REF1) and Small Rubber Particle Protein (SRPP1). Protein/lipid interactions were screened using two models (lipid vesicles in solution or lipid monolayers at air/liquid interface). Calcein leakage, surface pressure, ellipsometry, microscopy and spectroscopy revealed that both REF1 and SRPP1 displayed stronger interactions with anionic POPA and POPG, as compared to zwitterionic POPC. A particular behavior of REF1 was observed when interacting with POPA monolayers (i.e. aggregation + modification of secondary structure from α-helices to β-sheets, characteristic of its amyloid aggregated form), which might be involved in the irreversible coagulation mechanism of Hevea rubber particles.

Mots-clés Agrovoc : Hevea brasiliensis, latex, caoutchouc, acide gras, protéine, lipide, protéine synthétique, phosphatide, composition chimique

Mots-clés libres : Hevea brasiliensis, Rubber particle proteins, Rubber elongation factor, Small rubber particle protein, Anionic phospholipids, Latex coagulation

Classification Agris : F60 - Physiologie et biochimie végétale
Q60 - Traitement des produits agricoles non alimentaires

Champ stratégique Cirad : CTS 7 (2019-) - Hors champs stratégiques

Agences de financement hors UE : Agence Nationale de la Recherche, Kasetsart University, Ministère des Affaires Etrangères, Office of the Higher Education Commission, Agropolis Fondation

Projets sur financement : (FRA) De la particule de caoutchouc d'hévéa à la structure et aux propriétés du caoutchouc naturel: vers l'optimisation des performances du caoutchouc naturel

Auteurs et affiliations

• Wadeesirisak Kanthida, Kasetsart University (THA)
• Castano Sabine, CNRS (FRA)
• Vaysse Laurent, CIRAD-PERSYST-UPR BioWooEB (FRA)
• Bonfils Frédéric, CIRAD-PERSYST-UPR BioWooEB (FRA) ORCID: 0000-0001-7016-4770
• Peruch Frédéric, CNRS (FRA)
• Rattanaporn Kittipong, Kasetsart University (THA)
• Liengprayoon Siriluck, Kasetsart University (THA)
• Lecomte Sophie, CNRS (FRA) - auteur correspondant
• Bottier Céline, CIRAD-PERSYST-UPR BioWooEB (FRA) ORCID: 0000-0002-5318-4275 - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/606196/)

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