The genome of a bunyavirus cannot be defined at the level of the viral particle but only at the scale of the viral population

Yvon Michel, German Thomas L., Ullman Diane E., Dasgupta Ranjit, Parker Maxwell H., Ben-Mahmoud Sulley, Verdin Eric, Gognalons Patrick, Ancelin Aurélie, Laï Kee Him Joséphine, Girard Justine, Vernerey Marie-Stéphanie, Fernandez Emmanuel, Filloux Denis, Roumagnac Philippe, Bron Patrick, Michalakis Yannis, Blanc Stéphane. 2023. The genome of a bunyavirus cannot be defined at the level of the viral particle but only at the scale of the viral population. Proceedings of the National Academy of Sciences of the United States of America, 120 (48):e2309412120, 10 p.

https://doi.org/10.1073/pnas.2309412120

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Url - jeu de données - Entrepôt autre : https://www.ncbi.nlm.nih.gov/sra/PRJNA1020112

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Economie-gestion; Psychologie-éthologie-ergonomie

Résumé : Bunyaviruses are enveloped negative or ambisense single-stranded RNA viruses with a genome divided into several segments. The canonical view depicts each viral particle packaging one copy of each genomic segment in one polarity named the viral strand. Several opposing observations revealed nonequal ratios of the segments, uneven number of segments per virion, and even packaging of viral complementary strands. Unfortunately, these observations result from studies often addressing other questions, on distinct viral species, and not using accurate quantitative methods. Hence, what RNA segments and strands are packaged as the genome of any bunyavirus remains largely ambiguous. We addressed this issue by first investigating the virion size distribution and RNA content in populations of the tomato spotted wilt virus (TSWV) using microscopy and tomography. These revealed heterogeneity in viral particle volume and amount of RNA content, with a surprising lack of correlation between the two. Then, the ratios of all genomic segments and strands were established using RNA sequencing and qRT-PCR. Within virions, both plus and minus strands (but no mRNA) are packaged for each of the three L, M, and S segments, in reproducible nonequimolar proportions determined by those in total cell extracts. These results show that virions differ in their genomic content but together build up a highly reproducible genetic composition of the viral population. This resembles the genome formula described for multipartite viruses, with which some species of the order Bunyavirales may share some aspects of the way of life, particularly emerging properties at a supravirion scale.

Mots-clés Agrovoc : Orthobunyavirus, génome, virus des végétaux, variation génétique, arn, virologie

Mots-clés géographiques Agrovoc : France

Mots-clés libres : Bunyavirales, Arthotospovirus, Genome packaging, Multipartite virus, Genome formula

Classification Agris : H20 - Maladies des plantes

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Agences de financement hors UE : Université de Montpellier, Montpellier Université d'Excellence

Projets sur financement : (FRA) BLANC-MUSE2020-Multivir

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