Deep sequencing and variant frequency analysis for the quality control of a live bacterial vaccine against contagious bovine pleuropneumonia, strain T1

Thiaucourt François, Exbrayat Antoni, Loire Etienne, Boissiere Anne, Nwankpa Nick, Manso-Silvan Lucia. 2024. Deep sequencing and variant frequency analysis for the quality control of a live bacterial vaccine against contagious bovine pleuropneumonia, strain T1. Vaccine, 42 (8) : 1868-1872.

Url - jeu de données - Entrepôt autre : https://www.ncbi.nlm.nih.gov/bioproject/PRJNA1050060/

Résumé : Vaccination is the most cost-effective tool to control contagious bovine pleuropneumonia. The vaccines currently used in Africa are derived from a live strain called T1, which was attenuated by passage in embryonated eggs and broth culture. The number of passages is directly correlated to the degree of attenuation of the vaccinal strains and inversely correlated to their immunogenicity in cattle. Current quality control protocols applied to vaccine batches allow the assessment of identity, purity, and titers, but cannot assess the level of genetic drift form the parental vaccine strains. Deep sequencing was used to assess the genetic drift generated over controlled in vitro passages of the parental strain, as well as on commercial vaccine batches. Signatures of cloning procedures were detected in some batches, which imply a deviation from the standard production protocol. Deep sequencing is proposed as a new tool for the identity and stability control of T1 vaccines.

Mots-clés Agrovoc : péripneumonie contagieuse bovine, vaccin, vaccination, contrôle de qualité, pleuropneumonie, Mycoplasma mycoides

Mots-clés géographiques Agrovoc : Kenya

Mots-clés libres : Live bacterial vaccine, Quality control, Variant frequency analysis, Contagious bovine pleuropneumonia (CBPP), Mycoplasma mycoides subsp. mycoides (Mmm)

Classification Agris : L73 - Maladies des animaux

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Agences de financement hors UE : Bill and Melinda Gates Foundation, Foreign, Commonwealth and Development Office, Centre de Coopération Internationale en Recherche Agronomique pour le Développement

Auteurs et affiliations

• Thiaucourt François, Université de Montpellier (FRA)
• Exbrayat Antoni, CIRAD-BIOS-UMR ASTRE (FRA)
• Loire Etienne, CIRAD-BIOS-UMR ASTRE (FRA)
• Boissiere Anne, CIRAD-BIOS-UMR ASTRE (FRA)
• Nwankpa Nick, Pan African Veterinary Vaccine Centre (ETH)
• Manso-Silvan Lucia, CIRAD-BIOS-UMR ASTRE (FRA) ORCID: 0000-0002-5539-3804 - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/609501/)

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