Exploiting sorghum genetic diversity for enhanced aluminum tolerance: Allele mining based on the AltSB locus

Hufnagel Maciel Bárbara, Guimaraes Claudia Teixeira, Craft Eric J., Shaff Jon E., Schaffert Robert E., Kochian Leon V., Magalhaes Jurandir V.. 2018. Exploiting sorghum genetic diversity for enhanced aluminum tolerance: Allele mining based on the AltSB locus. Scientific Reports, 8:10094, 13 p.

https:/doi.org/10.1038/s41598-018-27817-z

Article de revue ; Article de recherche ; Article de revue à facteur d'impact Revue en libre accès total

Prévisualisation

Version publiée - Anglais
Sous licence

.
41598_2018_Article_27817.pdf
Télécharger (2MB) | Prévisualisation

Quartile : Q1, Sujet : MULTIDISCIPLINARY SCIENCES

Résumé : Root damage due to aluminum (Al) toxicity restricts crop production on acidic soils, which are extensive in the tropics. The sorghum root Al-activated citrate transporter, SbMATE, underlies the Al tolerance locus, AltSB, and increases grain yield under Al toxicity. Here, AltSB loci associated with Al tolerance were converted into Amplification Refractory Mutation System (ARMS) markers, which are cost effective and easy to use. A DNA pooling strategy allowed us to identify accessions harboring rare favorable AltSB alleles in three germplasm sets while greatly reducing genotyping needs. Population structure analysis revealed that favorable AltSB alleles are predominantly found in subpopulations enriched with guinea sorghums, supporting a possible Western African origin of AltSB. The efficiency of allele mining in recovering Al tolerance accessions was the highest in the largest and highly diverse germplasm set, with a 10-fold reduction in the number of accessions that would need to be phenotyped in the absence of marker information. Finally, Al tolerant accessions were found to rely on SbMATE to exclude Al3+ from sensitive sites in the root apex. This study emphasizes gene-specific markers as important tools for efficiently mining useful rare alleles in diverse germplasm, bridging genetic resource conservation efforts and pre-breeding for Al tolerance.

Mots-clés Agrovoc : marqueur génétique, Sorghum bicolor, génotype, conservation des ressources génétiques, germoplasme, industrie minière, sorgho en grain, allèle, collection de matériel génétique, diversité génétique (comme ressource), Sorghum, phénotype, toxicité

Mots-clés géographiques Agrovoc : Guinée

Mots-clés libres : Abiotic, Agricultural genetics, Genetic markers, Plant Breeding, Plant genetics

Agences de financement hors UE : Consortium of International Agricultural Research Centers, Empresa Brasileira de Pesquisa Agropecuária, Fundação de Amparo à Pesquisa do Estado de Minas Gerais, Conselho Nacional de Desenvolvimento Científico e Tecnológico

Auteurs et affiliations