Sorghum root epigenetic landscape during limiting phosphorus conditions

Gladman Nicholas, Hufnagel Maciel Bárbara, Regulski Michael, Liu Zhigang, Wang Xiaofei, Chougule Kapeel, Kochian Leon V., Magalhaes Jurandir V., Ware Doreen. 2022. Sorghum root epigenetic landscape during limiting phosphorus conditions. Plant Direct, 6 (5):e393, 17 p.

https://doi.org/10.1002/pld3.393

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Url - jeu de données - Entrepôt autre : https://www.ncbi.nlm.nih.gov/sra/?term=PRJNA454504

Résumé : Efficient acquisition and use of available phosphorus from the soil is crucial for plant growth, development, and yield. With an ever-increasing acreage of croplands with suboptimal available soil phosphorus, genetic improvement of sorghum germplasm for enhanced phosphorus acquisition from soil is crucial to increasing agricultural output and reducing inputs, while confronted with a growing world population and uncertain climate. Sorghum bicolor is a globally important commodity for food, fodder, and forage. Known for robust tolerance to heat, drought, and other abiotic stresses, its capacity for optimal phosphorus use efficiency (PUE) is still being investigated for optimized root system architectures (RSA). Whilst a few RSA-influencing genes have been identified in sorghum and other grasses, the epigenetic impact on expression and tissue-specific activation of candidate PUE genes remains elusive. Here, we present transcriptomic, epigenetic, and regulatory network profiling of RSA modulation in the BTx623 sorghum background in response to limiting phosphorus (LP) conditions. We show that during LP, sorghum RSA is remodeled to increase root length and surface area, likely enhancing its ability to acquire P. Global DNA 5-methylcytosine and H3K4 and H3K27 trimethylation levels decrease in response to LP, while H3K4me3 peaks and DNA hypomethylated regions contain recognition motifs of numerous developmental and nutrient responsive transcription factors that display disparate expression patterns between different root tissues (primary root apex, elongation zone, and lateral root apex).

Mots-clés Agrovoc : système racinaire, Sorghum bicolor, expression des gènes, morphologie végétale, phosphore disponible pour les plantes, Transcription génique, rhizosphère, carence minérale, tolérance à la sécheresse, phosphore, Sorghum

Mots-clés libres : Chromatin modification, DNA methylation, Histone marks, Histone methylation, Phosphorus efficiency, Phosphorus responsive genes, RNA seq, Root system architecture, Root system remodeling, Phosphorous deficiency

Classification Agris : F30 - Génétique et amélioration des plantes
F60 - Physiologie et biochimie végétale

Champ stratégique Cirad : CTS 2 (2019-) - Transitions agroécologiques

Agences de financement hors UE : Conselho Nacional de Desenvolvimento Científico e Tecnológico, Fundação de Amparo à Pesquisa do Estado de Minas Gerais, Canada Excellence Research Chairs, Government of Canada, University of Saskatchewan, U.S. Department of Agriculture

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