Identification of semiochemical candidates involved in Glossina Palpalis Gambiensis larviposition site selection and behavioural responses of adult gravid females

Gimonneau Geoffrey, Buatois Bruno, Lapeyre Benoit, Wendemanegde Salou Ernest, Sanon Nadège, Ranaivoarisoa Judith Annick, Roux Olivier, Dormont Laurent. 2024. Identification of semiochemical candidates involved in Glossina Palpalis Gambiensis larviposition site selection and behavioural responses of adult gravid females. Journal of Chemical Ecology, 14 p.

Url - jeu de données - Dataverse Cirad : https://doi.org/10.18167/DVN1/XZ8IAF

Résumé : Tsetse flies (Diptera: Glossinidae) are the cyclical vectors of human and animal trypanosomes. This viviparous insect develops and produces a single larva at 10-day intervals deposited in specific sites. In some species aggregation of larvae has been shown and seems to be mediated by both physical factors and volatile semiochemicals of larval origin. In this context, this study aims to identify chemicals emitted during the pupariation process in Glossina palpalis gambiensis. Volatile Organic Compounds (VOCs) emitted by larvae were identified using static headspace solid-phase microextraction and gas-chromatography mass-spectrometry (GC–MS) analysis. Electrophysiology and behavioural assays were performed on gravid females to confirm VOCs behavioural activity and attractiveness. GC–MS results revealed ten chemicals emitted during the pupariation process of G. p. gambiensis larvae. Among these chemicals, gravid females were shown to detect nine of them during coupled gas chromatography - electroantennographic detection tests. Behavioural assays highlighted two compounds were as attractive as pupae and one compound and a blend of four compounds were more attractive than pupae. Although the larval origin of some of them needs to be confirmed as they may also likely produced by micro-organisms, these compounds induced significant behavioural responses in the laboratory. Further experiments have to explore the biological activity and competitiveness of these compounds in the field. This work opens interesting opportunities for behavioural manipulation and control of tsetse flies.

Mots-clés Agrovoc : Glossina palpalis, Glossinidae, vecteur de maladie, comportement animal, transmission des maladies, larve, substance sémiochimique, Glossina

Mots-clés géographiques Agrovoc : Burkina Faso

Mots-clés complémentaires : Glossina palpalis gambiensis

Mots-clés libres : Tsetse fly, Reproduction, Chemical ecology, Larviposition, Aggregation

Classification Agris : L72 - Organismes nuisibles des animaux

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Agences de financement hors UE : Centre de Coopération Internationale en Recherche Agronomique pour le Développement

Projets sur financement : (FRA) Action incitative CRESI

Auteurs et affiliations

• Gimonneau Geoffrey, CIRAD-BIOS-UMR INTERTRYP (SEN) ORCID: 0000-0002-0613-841X - auteur correspondant
• Buatois Bruno, CEFE (FRA)
• Lapeyre Benoit, CEFE (FRA)
• Wendemanegde Salou Ernest, UPB (BFA)
• Sanon Nadège, CIRDES (BFA)
• Ranaivoarisoa Judith Annick, CIRAD-BIOS-UMR INTERTRYP (FRA)
• Roux Olivier, IRD (FRA)
• Dormont Laurent, CEFE (FRA)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/609696/)

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