Identification of a sugarcane bacilliform virus promoter that is activated by drought stress in plants

Sun Sheng-Ren, Wu Xiao-Bin, Chen Jian-Sheng, Huang Mei-Ting, Fu Hua-Ying, Wang Qin-Nan, Rott Philippe, Gao San-Ji. 2024. Identification of a sugarcane bacilliform virus promoter that is activated by drought stress in plants. Communications Biology, 7:368, 12 p.

Résumé : Sugarcane (Saccharum spp.) is an important sugar and biofuel crop in the world. It is frequently subjected to drought stress, thus causing considerable economic losses. Transgenic technology is an effective breeding approach to improve sugarcane tolerance to drought using drought-inducible promoter(s) to activate drought-resistance gene(s). In this study, six different promoters were cloned from sugarcane bacilliform virus (SCBV) genotypes exhibiting high genetic diversity. In β-glucuronidase (GUS) assays, expression of one of these promoters (PSCBV-YZ2060) is similar to the one driven by the CaMV 35S promoter and >90% higher compared to the other cloned promoters and Ubi1. Three SCBV promoters (PSCBV-YZ2060, PSCBV-TX, and PSCBV-CHN2) function as drought-induced promoters in transgenic Arabidopsis plants. In Arabidopsis, GUS activity driven by promoter PSCBV-YZ2060 is also upregulated by abscisic acid (ABA) and is 2.2–5.5-fold higher when compared to the same activity of two plant native promoters (PScRD29A from sugarcane and PAtRD29A from Arabidopsis). Mutation analysis revealed that a putative promoter region 1 (PPR1) and two ABA response elements (ABREs) are required in promoter PSCBV-YZ2060 to confer drought stress response and ABA induction. Yeast one-hybrid and electrophoretic mobility shift assays uncovered that transcription factors ScbZIP72 from sugarcane and AREB1 from Arabidopsis bind with two ABREs of promoter PSCBV-YZ2060. After ABA treatment or drought stress, the expression levels of endogenous ScbZIP72 and heterologous GUS are significantly increased in PSCBV-YZ2060:GUS transgenic sugarcane plants. Consequently, promoter PSCBV-YZ2060 is a possible alternative promoter for genetic engineering of drought-resistant transgenic crops such as sugarcane.

Mots-clés Agrovoc : Saccharum, génie génétique, expression des gènes, tolérance à la sécheresse, stress dû à la sécheresse, plante transgénique, mutation, Arabidopsis

Mots-clés géographiques Agrovoc : France

Mots-clés libres : Agricultural genetics, Drought

Classification Agris : H20 - Maladies des plantes
H50 - Troubles divers des plantes

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Agences de financement hors UE : Agriculture Research System of China, National Natural Science Foundation of China

Auteurs et affiliations

• Sun Sheng-Ren, Fujian Agriculture and Forestry University (CHN)
• Wu Xiao-Bin, Xiamen University (CHN)
• Chen Jian-Sheng, Fujian Agriculture and Forestry University (CHN)
• Huang Mei-Ting, Fujian Agriculture and Forestry University (CHN)
• Fu Hua-Ying, Fujian Agriculture and Forestry University (CHN)
• Wang Qin-Nan, Guangdong Academy of Agricultural Science (CHN)
• Rott Philippe, CIRAD-BIOS-UMR PHIM (FRA) ORCID: 0000-0001-6085-6159 - auteur correspondant
• Gao San-Ji, Fujian Agriculture and Forestry University (CHN) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/610071/)

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