Isothermal nucleic acid amplification to detect infection caused by parasites of the Trypanosomatidae family: A literature review and opinion on the laboratory to field applicability

Sereno Denis, Oury Bruno, Geiger Anne, Vela Andrea, Karmaoui Ahmed, Desquesnes Marc. 2022. Isothermal nucleic acid amplification to detect infection caused by parasites of the Trypanosomatidae family: A literature review and opinion on the laboratory to field applicability. International Journal of Molecular Sciences, 23 (14):7543, 22 p.

https://doi.org/10.3390/ijms23147543

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Résumé : Isothermal amplification of nucleic acids has the potential to be applied in resource-limited areas for the detection of infectious agents, as it does not require complex nucleic purification steps or specific and expensive equipment and reagents to perform the reaction and read the result. Since human and animal infections by pathogens of the Tryponasomatidae family occur mainly in resource-limited areas with scant health infrastructures and personnel, detecting infections by these methodologies would hold great promise. Here, we conduct a narrative review of the literature on the application of isothermal nucleic acid amplification for Trypanosoma and Leishmania infections, which are a scourge for human health and food security. We highlight gaps and propose ways to improve them to translate these powerful technologies into real-world field applications for neglected human and animal diseases caused by Trypanosomatidae.

Mots-clés Agrovoc : Trypanosoma cruzi, Trypanosoma, maladie des animaux, maladie de l'homme, adn, épidémiologie, maladie de Chagas, environnement socioéconomique, diagnostic, méthodologie, trypanosomose, Leishmania, identification, santé animale, matériel de laboratoire