O short'branch Microsporidia, where art thou? Identifying diversity hotspots for future sampling

Gross Megan, Rajter Ľubomír, Mahe Frédéric, Bass David, Berney Cédric, Henry Nicolas, de Vargas Colomban, Dunthorn Micah. 2024. O short'branch Microsporidia, where art thou? Identifying diversity hotspots for future sampling. European Journal of Protistology, 96:126119, 8 p.

https://doi.org/10.1016/j.ejop.2024.126119

Article de revue ; Article de recherche ; Article de revue à facteur d'impact

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Résumé : Short-branch Microsporidia were previously shown to form a basal grade within the expanded Microsporidia clade and to branch near the classical, long-branch Microsporidia. Although they share simpler versions of some morphological characteristics, they do not show accelerated evolutionary rates, making them ideal candidates to study the evolutionary trajectories that have led to long-branch microsporidian unique characteristics. However, most sequences assigned to the short-branch Microsporidia are undescribed, novel environmental lineages for which the identification requires knowledge of where they can be found. To direct future isolation, we used the EukBank database of the global UniEuk initiative that contains the majority of the publicly available environmental V4 SSU rRNA gene sequences of protists. The curated OTU table and corresponding metadata were used to evaluate the occurrence of short-branch Microsporidia across freshwater, hypersaline, marine benthic, marine pelagic, and terrestrial environments. Presence–absence analyses infer that short-branch Microsporidia are most abundant in freshwater and terrestrial environments, and alpha- and beta-diversity measures indicate that focusing our sampling effort on these two environments would cover a large part of their overall diversity. These results can be used to coordinate future isolation and sampling campaigns to better understand the enigmatic evolution of microsporidians' unique characteristics.

Mots-clés Agrovoc : milieu marin, biodiversité, identification, milieu benthique, Protista, milieu pélagique, microsporidiose

Mots-clés complémentaires : Microsporidia

Mots-clés libres : Metabarcoding, Parasites, Protists, SSU rRNA, V4 region

Classification Agris : M40 - Écologie aquatique
U60 - Sciences de la vie et de la Terre

Champ stratégique Cirad : CTS 1 (2019-) - Biodiversité

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