Development of microsatellite markers for the soft tick Ornithodoros phacochoerus

Taraveau Florian, Bru David, Quembo Carlos João, Jourdan-Pineau Hélène. 2024. Development of microsatellite markers for the soft tick Ornithodoros phacochoerus. Parasites and Vectors, 17:301, 9 p.

Résumé : Background: Soft ticks of the genus Ornithodoros are responsible for the maintenance and transmission of the African swine fever (ASF) virus in the sylvatic and domestic viral cycles in Southern Africa. They are also the main vectors of the Borrelia species causing relapsing fevers. Currently, no genetic markers are available for Afrotropical Ornithodoros ticks. As ASF spreads globally, such markers are needed to assess the role of ticks in the emergence of new outbreaks. The aim of this study is to design microsatellite markers that could be used for ticks of the Ornithodoros moubata complex, particularly Ornithodoros phacochoerus, to assess population structure and tick movements in ASF endemic areas. Methods: A total of 151 markers were designed using the O. moubata and O. porcinus genomes after elimination of repeated sequences in the genomes. All designed markers were tested on O. phacochoerus and O. porcinus DNA to select the best markers. Results: A total of 24 microsatellite markers were genotyped on two populations of O. phacochoerus and on individuals from four other Ornithodoros species. Nineteen markers were selected to be as robust as possible for population genetic studies on O. phacochoerus. Conclusions: The microsatellite markers developed here represent the first genetic tool to study nidicolous populations of O. phacochoerus.

Mots-clés Agrovoc : marqueur génétique, microsatellite, peste porcine africaine, génétique des populations, Ornithodoros moubata, virus peste porcine africaine, Ornithodoros, polymorphisme génétique, vecteur de maladie, épidémiologie, porcin, génotype, surveillance épidémiologique, parasite

Mots-clés géographiques Agrovoc : France

Mots-clés complémentaires : Ornithodoros phacochoerus

Mots-clés libres : Vector surveillance, Soft tick, Ornithodoros, Population genetics, Microsatellite markers, African swine fever

Classification Agris : L72 - Organismes nuisibles des animaux

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Agences de financement hors UE : National Institute of Food and Agriculture

Projets sur financement : (USA) Unraveling the effect of contact networks and socioeconomic factors in the emergence of infectious diseases at the wild‑domestic interface

Auteurs et affiliations

• Taraveau Florian, CIRAD-BIOS-UMR ASTRE (FRA) - auteur correspondant
• Bru David, INRAE (FRA)
• Quembo Carlos João, ARI (MOZ)
• Jourdan-Pineau Hélène, CIRAD-BIOS-UMR ASTRE (FRA) ORCID: 0000-0002-5118-9873 - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/611786/)

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