First investigation into the genetic control of meiosis in sugarcane

Reis Soares Nina, Portugal Costa Zirlane, Marques João Paulo Rodrigues, Garsmeur Olivier, Sampaio Carneiro Monalisa, Barros Monteiro Vitorello Cláudia, D'Hont Angélique, Carneiro Vieira Maria Lucia. 2024. First investigation into the genetic control of meiosis in sugarcane. The Plant Journal, 118 (6) : 2094-2107.

Résumé : The sugarcane (Saccharum spp.) genome is one of the most complex of all. Modern varieties are highly polyploid and aneuploid as a result of hybridization between Saccharum officinarum and S. spontaneum. Little research has been done on meiotic control in polyploid species, with the exception of the wheat Ph1 locus harboring the ZIP4 gene (TaZIP4-B2) which promotes pairing between homologous chromosomes while suppressing crossover between homeologs. In sugarcane, despite its interspecific origin, bivalent association is favored, and multivalents, if any, are resolved at the end of prophase I. Thus, our aim herein was to investigate the purported genetic control of meiosis in the parental species and in sugarcane itself. We investigated the ZIP4 gene and immunolocalized meiotic proteins, namely synaptonemal complex pro- teins Zyp1 and Asy1. The sugarcane ZIP4 gene is located on chromosome 2 and expressed more abundantly in flowers, a similar profile to that found for TaZIP4-B2. ZIP4 expression is higher in S. spontaneum a neoau- topolyploid, with lower expression in S. officinarum, a stable octoploid species. The sugarcane Zip4 protein contains a TPR domain, essential for scaffolding. Its 3D structure was also predicted, and it was found to be very similar to that of TaZIP4-B2, reflecting their functional relatedness. Immunolocalization of the Asy1 and Zyp1 proteins revealed that S. officinarum completes synapsis. However, in S. spontaneum and SP80-3280 (a modern variety), no nuclei with complete synapsis were observed. Importantly, our results have implications for sugarcane cytogenetics, genetic mapping, and genomics.

Mots-clés Agrovoc : Saccharum officinarum, Saccharum spontaneum, Saccharum, génome, méiose, polyploïdie, expression des gènes

Mots-clés libres : Diploidization, Immunolocalization of meiotic proteins, Meiotic control, Polyploidy, Saccharum, ZIP4 gene

Agences de financement hors UE : Fundação de Amparo à Pesquisa do Estado de São Paulo, Conselho Nacional de Desenvolvimento Científico e Tecnológico, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior

Auteurs et affiliations

• Reis Soares Nina, USP (BRA)
• Portugal Costa Zirlane, USP (BRA)
• Marques João Paulo Rodrigues, USP (BRA)
• Garsmeur Olivier, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0001-8869-3689
• Sampaio Carneiro Monalisa, UFSCar (BRA)
• Barros Monteiro Vitorello Cláudia, USP (BRA)
• D'Hont Angélique, CIRAD-BIOS-UMR AGAP (FRA)
• Carneiro Vieira Maria Lucia, USP (BRA) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/612920/)

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