Characterization and PCR multiplexing of polymorphic microsatellite loci for the locust Locusta migratoria

Chapuis Marie-Pierre, Loiseau Anne, Michalakis Yannis, Lecoq Michel, Estoup Arnaud. 2005. Characterization and PCR multiplexing of polymorphic microsatellite loci for the locust Locusta migratoria. Molecular Ecology Notes, 5 (3) : 554-557.

Note générale : Corrigendum paru dans Molecular Ecology Resources, 10 (4) : 755, https://doi.org/10.1111/j.1755-0998.2010.02870.x

Résumé : Because of the scarcity of polymorphic genetic markers available in locust species, only a few population genetics studies have been carried out on this taxon. We isolated and characterized 11 polymorphic microsatellite loci in the pest locust Locusta migratoria capito, and described experimental conditions for polymerase chain reaction (PCR) multiplexing and simultaneously genotyping these loci. The number of alleles per locus ranged from six to 25, and the expected heterozygosity ranged from 0.431 to 0.957. Results of cross-taxon amplification tests are reported in six other Locusta migratoria subspecies, six species of the Oedipodinae subfamily and two other pest locust species.

Mots-clés Agrovoc : Locusta migratoria, microsatellite, locus, génotype, identification, marqueur génétique, PCR

Classification Agris : H10 - Ravageurs des plantes

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

• Chapuis Marie-Pierre, INRA (FRA)
• Loiseau Anne, INRA (FRA)
• Michalakis Yannis, CNRS (FRA)
• Lecoq Michel, CIRAD-AMIS-UPR Acridologie (FRA)
• Estoup Arnaud, INRA (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/530018/)

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