Montoya Carmenza, Cuellar Térésa, Lopes Ricardo, Summo Marilyne, Bittencourt Daniela, Pinaglia Céline, Rivallan Ronan, Espeout Sandra, Pitollat Bertrand, Flori Albert, Villeneuve Pierre, Pina Michel, Cochard Benoît, Cros David, Herrero Javier, Omoré Alphonse, Zambrano Jorge, Syaputra Indra, Alarcón Wilmar Hernán, Nouy Bruno, Amblard Philippe, Leroy Thierry, Ritter Enrique, Billotte Norbert.
2014. Genetic determinism of the fatty acid composition of palm oil in Elaeis (E. guineensis Jacq. and E. oleifera (H.B.K) Cortès) by genetic mapping and differential expression analysis of candidate genes : [W550].
In : Plant and Animal Genomes Conference XXII Conference, San diego, United States, San Diego, United States, January 11-15, 2014
|
Version publiée
- Anglais
Utilisation soumise à autorisation de l'auteur ou du Cirad. document_573284.pdf Télécharger (55kB) | Prévisualisation |
Résumé : In this study of the International Oil Palm Genome Projects (OPGP) Consortium, an Elaeis interspecific pseudo-backcross of first generation (E. oleifera x E. guineensis) x E. guineensis allowed tracing segregating alleles from both Elaeis genomes. Nineteen quantitative trait loci (QTLs) associated to palm oil fatty acid proportions compared to 16 QTLs of same traits in an intra-specific oil palm cross evidenced common or specific QTLs in E. guineensis and E. oleifera. Genes related to palm oil fatty acids were investigated using full-length cDNA libraries and mRNA extracts from the mesocarp of developing fruits in each Elaeis species represented by four genetic pools. Expression of 113 key Elaeis genes or putative regulation factors of de novo fatty acid and triacylglycerol pathways were characterized at species level by in silico transcript profiling and Real Time RT-qPCR analysis. Genes were mapped on the pseudo-backcross using 180 intra-gene SNP markers. Forty-eight genes were differentially expressed between Elaeis species, with 94% over expressed in E. guineensis. The EgAP2-2 (WRI1-like) transcription factor might influence the start of the de novo fatty acid pathway by main action only on the MAT and KAS III genes, as independent in expression from other genes and not differential between species. Between species variations in C16:0 and C18:1 can be deciphered by relative expression levels and enzyme activities of the isoforms in the genome of FATB (2), KAS II (1) and SAD (3). (Texte intégral)
Classification Agris : F30 - Génétique et amélioration des plantes
F60 - Physiologie et biochimie végétales
Q04 - Composition des produits alimentaires
Auteurs et affiliations
- Montoya Carmenza, CENIPALMA (COL)
- Cuellar Térésa, CIRAD-BIOS-UMR AGAP (FRA)
- Lopes Ricardo, EMBRAPA (BRA)
- Summo Marilyne, CIRAD-BIOS-UMR AGAP (FRA)
- Bittencourt Daniela, EMBRAPA (BRA)
- Pinaglia Céline
- Rivallan Ronan, CIRAD-BIOS-UMR AGAP (FRA)
- Espeout Sandra, CIRAD-BIOS-UMR AGAP (FRA)
- Pitollat Bertrand, CIRAD-BIOS-UMR AGAP (FRA)
- Flori Albert, CIRAD-BIOS-UMR AGAP (FRA)
-
Villeneuve Pierre, CIRAD-PERSYST-UMR IATE (FRA)
ORCID: 0000-0003-1685-1494
- Pina Michel, CIRAD-PERSYST-UMR IATE (FRA)
- Cochard Benoît, CIRAD-BIOS-UMR AGAP (FRA)
-
Cros David, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0002-8601-7991
- Herrero Javier, NEIKER (ESP)
- Omoré Alphonse, INRAB (BEN)
- Zambrano Jorge, Hacienda La Cabaña (COL)
- Syaputra Indra, SOCFINDO (IDN)
- Alarcón Wilmar Hernán, Indupalma (COL)
- Nouy Bruno, CIRAD-BIOS-UMR AGAP (FRA)
- Amblard Philippe, CIRAD-BIOS-UMR AGAP (FRA)
- Leroy Thierry, CIRAD-BIOS-UMR AGAP (FRA)
- Ritter Enrique, NEIKER (ESP)
- Billotte Norbert, CIRAD-BIOS-UMR AGAP (FRA)
Source : Cirad - Agritrop (https://agritrop.cirad.fr/573284/)
[ Page générée et mise en cache le 2019-09-30 ]
