Development of a specific molecular tool for the detection of epidemiologically active mulberry causing-disease strains of Ralstonia solanacearum phylotype I (historically race 5-biovar 5) in China

Pan Z.C., Xu Jianchu, Prior Philippe, Xu J.S., Zhang H., Chen K.Y., Tian Q., Zhang L.Q., Liu L., He L.Y., Feng J.. 2013. Development of a specific molecular tool for the detection of epidemiologically active mulberry causing-disease strains of Ralstonia solanacearum phylotype I (historically race 5-biovar 5) in China. European Journal of Plant Pathology, 137 (2) : 377-391.

Quartile : Q1, Sujet : HORTICULTURE / Quartile : Q2, Sujet : AGRONOMY / Quartile : Q2, Sujet : PLANT SCIENCES

Résumé : Ralstonia solanacearum causes bacterial wilt disease in many plant species, including mulberry. Here, we used a suppression subtractive hybridization (SSH) approach to identify specific DNA fragments in R. solanacearum race 5-biovar 5. The genome of the R. solanacearum M7 strain was subtracted from that of the GMI1000 strain, resulting in the identification of 85 subtracted fragments. The primer set MG67-F/R for identification of Ralstonia solanacearum race 5-biovar 5 strains was designed on the basis of the clone MG67 sequence. Furthermore, a multiplex PCR was developed by using the primer set MG67-F/MG67R in combination with the species-specific primer pair 759/760. A 156 bp r5-bv5-specific fragment, together with a 282 bp species-specific fragment, was amplified from all tested R. solanacearum r5-bv5 strains. The sensitivity of the multiplex PCR made it possible to detect concentrations as low as 102 CFU ml−1 of pure culture. Moreover, the r5-bv5-specific multiplex PCR was successfully applied to detect Ralstonia solanacearum race 5-biovar 5 strains in diseased mulberry samples. Therefore, the multiplex PCR assay can be used as a reliable diagnostic technique to enable researchers to rapidly identify isolates of R. solanacearum race 5-biovar 5.

Mots-clés Agrovoc : Ralstonia solanacearum, biologie moléculaire, identification, hybridation, épidémiologie, technique analytique, PCR, Broussonetia papyrifera

Mots-clés géographiques Agrovoc : Chine

Classification Agris : H20 - Maladies des plantes

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

• Pan Z.C., CAAS (CHN)
• Xu Jianchu, CAS (CHN)
• Prior Philippe, CIRAD-BIOS-UMR PVBMT (REU)
• Xu J.S., CAAS (CHN)
• Zhang H., CAAS (CHN)
• Chen K.Y., CAAS (CHN)
• Tian Q., CAAS (CHN)
• Zhang L.Q., CAAS (CHN)
• Liu L., CAAS (CHN)
• He L.Y., CAAS (CHN)
• Feng J., CAAS (CHN)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/587410/)

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