Regulation of Redox-Related Genes in Hevea Brasiliensis and Their Role in Latex Production

Montoro Pascal, Zhang Yi, Martin Florence, Kuswanhadi, Putranto Riza Arief, Leclercq Julie. 2018. Regulation of Redox-Related Genes in Hevea Brasiliensis and Their Role in Latex Production. In : Comparative Genomics and Interactomics for Agriculture. Institute of plantation studies (IKP), Universiti Putra Malaysia. Selangor : IKP, Résumé, 27. ICCGIA 2018. International Conference on Comparative Genomics and Interactomics for Agriculture, Selangor, Malaisie, 1 Novembre 2018/2 Novembre 2018.

Note générale : Pascal Montoro est l'expert invité du Cirad

Résumé : Hevea brasiliensis is the main source of natural rubber accounting for 42% of the worldwide rubber consumption. Natural rubber is synthesized in rubber particles of latex cells, which are differentiated from the vascular cambia and localized in the seconda:ry phloem. Latex is collected by tapping the soft bark of rubber trees. Ethephon, an ethylene releaser, is applied on bark to stimulate latex flow and regeneration between two tappings. Above a certain threshold, environmental and harvesting stresses are known to induce an oxidative stress triggering Tapping Panel Dryness (TPD). TPD is a physiological syndrome affecting latex production through the agglutination of rubber particles. Four hundred and seven genes from 30 gene families related to reactive oxygen species (ROS) production and scavenging, and antioxidants biosynthesis were identified in the Hevea genome sequence. Based on a transcriptome analysis, 161 ROS-related genes were found expressed in latex cells. Small RNA and degradome analysis revealed 13 genes targeted by 11 microRNAs and 15 genes targeted by 16 transactingsiRNA in latex. These post-transcriptional regulations dramatically affect their gene expression profile. HbRBOH2 was identified as the main source gene of ROS in latex, while HbCuZnSOD4 might be the most important ROS scavenging enzyme for ROS detoxification in latex. Overexpression of genes encoding a superoxide dismutase (HbCuZnSOD) and an enzyme of the glutathione biosynthetic pathway (EcGSHl ) were successfully obtained in transgenic rubber plants. These later showed an increase in plant growth and their tolerance to abiotic stress. This study revealed the crucial role of antioxidant in Hevea brasiliensis laticifers and suggests seeking genetic variability for antioxidant capacity in order to improve rubber tree for the tolerance to abiotic stress and TPD occurrence.

Mots-clés libres : Hevea brasiliensis, Redox, Antioxidant, Ethylene, Latex

Auteurs et affiliations

• Montoro Pascal, CIRAD-BIOS-UMR AGAP (FRA)
• Zhang Yi, CIRAD-BIOS-UMR AGAP (FRA)
• Martin Florence, CIRAD-BIOS-UMR AGAP (FRA)
• Kuswanhadi, IRRI [Indonesian Rubber Research Institute] (IDN)
• Putranto Riza Arief, Indonesian Research Institute for Biotechnology and Bioindustry (IDN)
• Leclercq Julie, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0001-8110-3762

Source : Cirad-Agritrop (https://agritrop.cirad.fr/589540/)

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