Quantitative proteomic analysis of the sugarcane defense responses incited by Acidovorax avenae subsp. avenae causing red stripe

Zhou Jing-Ru, Sun Hui-Dong, Ali Ahmed, Rott Philippe, Javed Talha, Fu Hua-Ying, Gao San-Ji. 2021. Quantitative proteomic analysis of the sugarcane defense responses incited by Acidovorax avenae subsp. avenae causing red stripe. Industrial Crops and Products, 162:113275, 11 p.

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Abstract : The causal agent of red stripe, Acidovorax avenae subsp. avenae (Aaa), can incite significant economic losses during sugarcane production. Proteomics is a useful tool for the comprehensive identification of defense response proteins in plants. In this study, isobaric tags for relative and absolute quantification (iTRAQ)-based proteomics was used to identify proteins that were produced in sugarcane in defense to Aaa in two Saccharum spp. cultivars: ROC22 (resistant to red stripe) and MT11−610 (susceptible). Defense response was evaluated at 0 h (mock-inoculation) and 24 h after inoculation with the bacterial pathogen, and 10,502 high-confidence proteins and 1962 differentially expressed proteins (DEPs) were identified. These DEPs included 1027 proteins (671 upregulated, 354 downregulated) in ROC22 and 1130 proteins (566 upregulated, 564 downregulated) in MT11−610. Gene ontology analysis revealed that most DEPs were clustered in the metabolic, single-organism metabolic, and oxidation-reduction processes, and in catalytic activity. In a KEGG analysis, these proteins belonged to common pathways such as secondary metabolite biosynthesis and biosynthesis of phenylpropanoids. Thirty-six DEPs were identified, especially epoxide hydrolase 1, dynamin-related protein 1C-like, cytochrome oxidase subunit I, FK506-binding protein, acetoacetyl CoA thiolase isoform 2, anthocyanidin 3-o-glucosyltransferase, ubiquitin carboxyl-terminal hydrolase 4, protein disulfide isomerase-like protein, and jumonji-like transcription factor family protein. These nine proteins were characterized by enhanced expression with ≥1.5-fold increases in resistant ROC22 versus susceptible MT11−610, and are therefore biomarker candidates in the sugarcane response against Aaa. These data could be very useful in molecular breeding for sugarcane resistance to red stripe.

Mots-clés Agrovoc : Maladie des plantes, protéomique, Analyse quantitative, Réponse de la plante, mécanisme de défense, Saccharum, Résistance aux maladies

Mots-clés complémentaires : Acidovorax avenae

Mots-clés libres : Acidovorax avenae subsp. avenae, Differentially expressed proteins, ITRAQ, Proteomics, Red stripe, Saccharum spp.

Classification Agris : H20 - Plant diseases

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Auteurs et affiliations

  • Zhou Jing-Ru, Fujian Agriculture and Forestry University (CHN)
  • Sun Hui-Dong, Fujian Agriculture and Forestry University (CHN)
  • Ali Ahmed, Fujian Agriculture and Forestry University (CHN)
  • Rott Philippe, CIRAD-BIOS-UMR PHIM (FRA) ORCID: 0000-0001-6085-6159
  • Javed Talha, Fujian Agriculture and Forestry University (CHN)
  • Fu Hua-Ying, Fujian Agriculture and Forestry University (CHN)
  • Gao San-Ji, Fujian Agriculture and Forestry University (CHN) - auteur correspondant

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