Genomic instability in somatic hybridization between Poncirus and Citrus species aiming to create new rootstocks

Dambier Dominique, Barantin Pascal, Boulard Gabriel, Costantino Gilles, Mournet Pierre, Perdereau Aude, Morillon Raphaël, Ollitrault Patrick. 2022. Genomic instability in somatic hybridization between Poncirus and Citrus species aiming to create new rootstocks. Agriculture (Basel), 12 (2):134, 26 p.

Url - jeu de données - Entrepôt autre : https://www.ncbi.nlm.nih.gov/bioproject/PRJNA648274 / Url - jeu de données - Entrepôt autre : https://www.ncbi.nlm.nih.gov/bioproject/PRJNA794128

Résumé : Rootstocks are an important component for citrus adaptation to increasing biotic and abiotic stresses resulting from global climate change. There is a strong complementarity between Citrus species, which adapt to abiotic stresses, and Poncirus trifoliata and its intergeneric hybrids, which exhibit resistances or tolerances to major diseases and pests. Thus, symmetrical somatic hybridization between complementary diploid rootstocks of these two genera appears to be an efficient way to develop new tetraploid rootstocks in order to address the new challenges of the citrus industry. New intergeneric somatic hybrids were obtained by electrofusion between protoplasts of Citrus and P. trifoliata hybrids. Extensive characterization of the nuclear and cytoplasmic genomes was performed by genotyping-by-sequencing (GBS) analysis. This revealed diploid cybrids and nuclear somatic hybrids. Mitochondrial genomes were mostly inherited from the callus parent, but homologous recombination events were observed for one parental combination. Chloroplasts exhibited random uniparental inheritance. GBS revealed local chromosomal instabilities for all nuclear somatic hybrids and whole chromosome eliminations for two hybrids. However, at the whole genome level, symmetrical addition of the nuclear genomes of both parents was predominant and all somatic hybrids displayed at least one trifoliate orange haplotype throughout the genome.

Mots-clés Agrovoc : amélioration des plantes, porte greffe, résistance aux facteurs nuisibles, hybridation somatique, stress abiotique, stress biotique, résistance physiologique au stress, génomique, Poncirus trifoliata, Citrus

Mots-clés libres : Protoplast fusion, Somatic embryogenesis, Genotyping by sequencing, Somaclonal variation, Citrus sinensis, Citrus reticulata, Poncirus trifoliata, Citrange, Citrumelo, Citrandarin

Classification Agris : F30 - Génétique et amélioration des plantes
H01 - Protection des végétaux - Considérations générales

Champ stratégique Cirad : CTS 2 (2019-) - Transitions agroécologiques

Auteurs et affiliations

• Dambier Dominique, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0002-9117-8853
• Barantin Pascal, CIRAD-BIOS-UMR AGAP (FRA)
• Boulard Gabriel, INRAE (FRA)
• Costantino Gilles, INRAE (FRA)
• Mournet Pierre, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0001-8011-8647
• Perdereau Aude, Genoscope (FRA)
• Morillon Raphaël, CIRAD-BIOS-UMR AGAP (FRA)
• Ollitrault Patrick, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0002-9456-5517 - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/600069/)

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