NIN-like protein transcription factors regulate leghemoglobin genes in legume nodules

Jiang Suyu, Jardinaud Marie-Françoise, Gao Jinpeng, Pecrix Yann, Wen Jiangqi, Mysore Kirankumar, Xu Ping, Sanchez-Canizares Carmen, Ruan Yiting, Li Qiujiu, Zhu Meijun, Li Fuyu, Wang Ertao, Poole Phillip S., Gamas Pascal, Murray Jeremy D.. 2021. NIN-like protein transcription factors regulate leghemoglobin genes in legume nodules. Science, 374 (6567) : 625-628.

Quartile : Outlier, Sujet : MULTIDISCIPLINARY SCIENCES

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Anthropologie-Ethnologie; Economie-gestion; Psychologie-éthologie-ergonomie; Sociologie-démographie

Résumé : Leghemoglobins enable the endosymbiotic fixation of molecular nitrogen (N2) in legume nodules by channeling O2 for bacterial respiration while maintaining a micro-oxic environment to protect O2-sensitive nitrogenase. We found that the NIN-like protein (NLP) transcription factors NLP2 and NIN directly activate the expression of leghemoglobins through a promoter motif, resembling a “double” version of the nitrate-responsive elements (NREs) targeted by other NLPs, that has conserved orientation and position across legumes. CRISPR knockout of the NRE-like element resulted in strongly decreased expression of the associated leghemoglobin. Our findings indicate that the origins of the NLP-leghemoglobin module for O2 buffering in nodules can be traced to an ancient pairing of NLPs with nonsymbiotic hemoglobins that function in hypoxia.

Mots-clés Agrovoc : nodosité racinaire, transcription, facteur de transcription, léghémoglobine, légumineuse, Fixation de l'azote, Medicago truncatula

Mots-clés complémentaires : Lotus japonicus

Classification Agris : F30 - Génétique et amélioration des plantes

Champ stratégique Cirad : CTS 2 (2019-) - Transitions agroécologiques

Auteurs et affiliations

• Jiang Suyu, Shanghai Institute of Plant Physiology and Ecology (CHN)
• Jardinaud Marie-Françoise, Université de Toulouse (FRA)
• Gao Jinpeng, Shanghai Institute of Plant Physiology and Ecology (CHN)
• Pecrix Yann, CIRAD-BIOS-UMR PVBMT (REU) ORCID: 0000-0002-6537-3145
• Wen Jiangqi, Noble Research Institute (USA)
• Mysore Kirankumar, Noble Research Institute (USA)
• Xu Ping, Shanghai Normal University (CHN)
• Sanchez-Canizares Carmen, University of Oxford (GBR)
• Ruan Yiting, Shanghai Institute of Plant Physiology and Ecology (CHN)
• Li Qiujiu, Shanghai Institute of Plant Physiology and Ecology (CHN)
• Zhu Meijun, Shanghai Institute of Plant Physiology and Ecology (CHN)
• Li Fuyu, Shanghai Institute of Plant Physiology and Ecology (CHN)
• Wang Ertao, Shanghai Institute of Plant Physiology and Ecology (CHN)
• Poole Phillip S., University of Oxford (GBR)
• Gamas Pascal, Université de Toulouse (FRA)
• Murray Jeremy D., Shanghai Institute of Plant Physiology and Ecology (CHN) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/600455/)

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