Application of deep eutectic solvents in protein extraction and purification

Bowen Hou, Durrani Rabia, Delavault André, Durand Erwann, Chenyu Jiang, Yiyang Long, Lilia Song, Jian Song, Weiwei Huan, Fei Gao. 2022. Application of deep eutectic solvents in protein extraction and purification. Frontiers in Chemistry, 10:912411, 10 p.

Résumé : Deep eutectic solvents (DESs) are a mixture of hydrogen bond donor (HBD) and hydrogen bond acceptor (HBA) molecules that can consist, respectively, of natural plant metabolites such as sugars, carboxylic acids, amino acids, and ionic molecules, which are for the vast majority ammonium salts. Media such as DESs are modular tools of sustainability that can be pointed toward the extraction of bioactive molecules due to their excellent physicochemical properties, their relatively low price, and accessibility. The present review focuses on the application of DESs for protein extraction and purification. The in-depth effects and principles that apply to DES-mediated extraction using various renewable biomasses will be discussed as well. One of the most important observations being made is that DESs have a clear ability to maintain the biological and/or functional activity of the extracted proteins, as well as increase their stability compared to traditional solvents. They demonstrate true potential for a reproducible but more importantly, scalable protein extraction and purification compared to traditional methods while enabling waste valorization in some particular cases.

Mots-clés Agrovoc : extraction par solvant, purification, solvant, protéine, biodégradabilité, protéine animale, protéine végétale, utilisation des déchets, propriété physicochimique

Mots-clés complémentaires : solvant eutectique profond

Mots-clés libres : Deep eutectic solvents, Protein, Extraction, Green solvent, Biodegradability

Classification Agris : U50 - Sciences physiques et chimie

Champ stratégique Cirad : CTS 7 (2019-) - Hors champs stratégiques

Auteurs et affiliations

• Bowen Hou, Zhejiang Agriculture and Forestry University (CHN)
• Durrani Rabia, Zhejiang Agriculture and Forestry University (CHN)
• Delavault André, Karlsruhe Institute of Technology (DEU)
• Durand Erwann, CIRAD-PERSYST-UMR Qualisud (FRA) ORCID: 0000-0002-0306-8081
• Chenyu Jiang, Zhejiang Agriculture and Forestry University (CHN)
• Yiyang Long, Zhejiang Agriculture and Forestry University (CHN)
• Lilia Song, Zhejiang Agriculture and Forestry University (CHN)
• Jian Song, Zhejiang Agriculture and Forestry University (CHN)
• Weiwei Huan, Zhejiang Agriculture and Forestry University (CHN) - auteur correspondant
• Fei Gao, Zhejiang Agriculture and Forestry University (CHN) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/601917/)

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