NIRS acquisition on fresh cassava roots using the ASD Quality Spec (QST) and relating spectra to root dry matter content by oven method. High-throughput phenotyping protocols (HTPP), WP3

Nuwamanya Ephraim, Kanaabi Michael, Alamu Emmanuel Oladeji, Meghar Karima. 2021. NIRS acquisition on fresh cassava roots using the ASD Quality Spec (QST) and relating spectra to root dry matter content by oven method. High-throughput phenotyping protocols (HTPP), WP3. Kampala : RTBfoods Project-CIRAD, 10 p.

Url - jeu de données - Dataverse Cirad : https://doi.org/10.18167/DVN1/AIKPJV / Url - jeu de données - Dataverse Cirad : https://doi.org/10.18167/DVN1/OOVUVL

Résumé : The Standard Operating Procedures (SOPs) detailed herein are applicable in the acquisition of spectra from largely intact cassava roots. The procedures allow for scanning and spectral acquisition after a scanning surface has been provided on the intact root. Cassava samples are harvested using approved harvesting tools and labelled after which preparation commences by removing the tail ends of the root. This is followed by sectioning of the root into four equal portions and surface trimming of each section immediately before spectral acquisition. Thereafter, labelling and scanning of the trimmed surface using the ASD-QUALITY SPEC produces four scans representative of one particular root. The procedure is repeated for the rest of the roots producing four subsets of scans from one particular accession. Spectral data produced from these scans is downloaded and further processed for use in calibration development and root chemical composition determination. Processed spectral data is also uploaded as an additional file to the cassava base. Reference data generation is carried out by approved reference methods in repeatability analyses carried out at NaCRRI and NARL. Critical points of consideration include the development of a sample flow and spectra acquisition matrix coupled to correct labelling since sample numbers involved are usually many.

Mots-clés libres : Cassava, Spectra, Procedure, Reference data, Repeatability

Agences de financement hors UE : Bill and Melinda Gates Foundation, Centre de Coopération Internationale en Recherche Agronomique pour le Développement, Centro Internacional de Agricultura Tropical, Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement, James Hutton Institute

Projets sur financement : (FRA) Breeding RTB Products for End User Preferences

Auteurs et affiliations

• Nuwamanya Ephraim, NaCRRI (UGA)
• Kanaabi Michael, NaCRRI (UGA)
• Alamu Emmanuel Oladeji, IITA (NGA)

Contributeurs et affiliations

• Meghar Karima, CIRAD-PERSYST-UMR Qualisud (FRA) - collaborateur

Source : Cirad-Agritrop (https://agritrop.cirad.fr/602134/)

Métriques

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