Improved growth and tuber quality of transgenic potato plants overexpressing either NHX antiporter, CLC chloride channel, or both

Ayadi Mariem, Chiab Nour, Charfeddine Safa, Abdelhedi Rania, Dabous Amira, Talbi Ons, Mieulet Delphine, Guiderdoni Emmanuel, Aifa Sami, Gargouri-Bouzid Radhia, Hanana Mohsen. 2022. Improved growth and tuber quality of transgenic potato plants overexpressing either NHX antiporter, CLC chloride channel, or both. Plant Physiology and Biochemistry, 189 : 45-58.

Résumé : The nutritional enhancement of potato plants (Solanum tuberosum L.,) is highly critical. As it is considered a worldwide basic vegetarian nutrition to maintain health. S. tuberosum is one of the foremost staples and the world's fourth-largest food crop. In advance, its need is increasing because of its high-industrial value and population blast. To improve both potato growth and behavior under harsh environmental conditions, we produced transgenic potato plants overexpressing either VvNHX (a sodium proton antiporter from Vitis vinifera), VvCLC (a chloride channel from Vitis vinifera), or both. Control and transgenic plants were grown in greenhouse and field under non-stressed conditions for 85 days in order to characterize their phenotype and evaluate their agronomical performance. To this aim, the evaluation of plant growth parameters, tuber yields and characteristics (calibers, eye number and color), the chemical composition of tubers, was conducted and compared between the different lines. The obtained results showed that transgenic plants displayed an improved growth (flowering precocity, gain of vigor and better vegetative growth) along with enhanced tuber yields and quality (increased protein and starch contents). Our findings provide then insight into the role played by the VvNHX antiport and the VvCLC channel and a greater understanding of the effect of their overexpression in potato plants.

Mots-clés Agrovoc : Solanum tuberosum, Vitis vinifera, plante transgénique, croissance, santé des plantes, antiport, tubercule, nutrition des plantes, chlorure, anatomie végétale, phénotype, pomme de terre

Mots-clés libres : Potato, Trangenic, Antiporter, NHX

Classification Agris : F30 - Génétique et amélioration des plantes

Champ stratégique Cirad : CTS 2 (2019-) - Transitions agroécologiques

Agences de financement hors UE : Agropolis Fondation

Projets sur financement : (FRA) RicE FUnctional GEnomics

Auteurs et affiliations

• Ayadi Mariem, University of Sfax (TUN)
• Chiab Nour, Ecole nationale d'ingénieurs de Sfax (TUN)
• Charfeddine Safa, Ecole nationale d'ingénieurs de Sfax (TUN)
• Abdelhedi Rania, University of Sfax (TUN)
• Dabous Amira, CBBC [Centre of Biotechnology of Borj-Cédria] (TUN)
• Talbi Ons, CBBC [Centre of Biotechnology of Borj-Cédria] (TUN)
• Mieulet Delphine, CIRAD-BIOS-UMR DIADE (FRA) ORCID: 0000-0001-6220-0372
• Guiderdoni Emmanuel, CIRAD-BIOS-UMR AGAP (FRA)
• Aifa Sami, University of Sfax (TUN)
• Gargouri-Bouzid Radhia, Ecole nationale d'ingénieurs de Sfax (TUN)
• Hanana Mohsen, CBBC [Centre of Biotechnology of Borj-Cédria] (TUN) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/604958/)

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