A multi-level approach reveals key physiological and molecular traits in the response of two rice genotypes subjected to water deficit at the reproductive stage

Favreau Bénédicte, Gaal Camille, Pereira De Lima Isabela, Droc Gaëtan, Roques Sandrine, Sotillo Armelle, Guérard Florence, Cantonny Valérie, Gakière Bertrand, Leclercq Julie, Lafarge Tanguy, De Raissac Marcel. 2023. A multi-level approach reveals key physiological and molecular traits in the response of two rice genotypes subjected to water deficit at the reproductive stage. Plant-Environment Interactions, 4 (5) : 229-257.

Résumé : Rice is more vulnerable to drought than maize, wheat, and sorghum because its water requirements remain high throughout the rice life cycle. The effects of drought vary depending on the timing, intensity, and duration of the events, as well as on the rice genotype and developmental stage. It can affect all levels of organization, from genes to the cells, tissues, and/or organs. In this study, a moderate water deficit was applied to two contrasting rice genotypes, IAC 25 and CIRAD 409, during their reproductive stage. Multi-level transcriptomic, metabolomic, physiological, and morphological analyses were performed to investigate the complex traits involved in their response to drought. Weighted gene network correlation analysis was used to identify the specific molecular mechanisms regulated by each genotype, and the correlations between gene networks and phenotypic traits. A holistic analysis of all the data provided a deeper understanding of the specific mechanisms regulated by each genotype, and enabled the identification of gene markers. Under non-limiting water conditions, CIRAD 409 had a denser shoot, but shoot growth was slower despite better photosynthetic performance. Under water deficit, CIRAD 409 was weakly affected regardless of the plant level analyzed. In contrast, IAC 25 had reduced growth and reproductive development. It regulated transcriptomic and metabolic activities at a high level, and activated a complex gene regulatory network involved in growth-limiting processes. By comparing two contrasting genotypes, the present study identified the regulation of some fundamental processes and gene markers, that drive rice development, and influence its response to water deficit, in particular, the importance of the biosynthetic and regulatory pathways for cell wall metabolism. These key processes determine the biological and mechanical properties of the cell wall and thus influence plant development, organ expansion, and turgor maintenance under water deficit. Our results also question the genericity of the antagonism between morphogenesis and organogenesis observed in the two genotypes.

Mots-clés Agrovoc : Oryza sativa, physiologie de la reproduction, génotype, stade de développement végétal, expression des gènes, physiologie végétale, résistance à la sécheresse, gène, croissance, morphologie végétale, résistance génétique, paroi cellulaire, développement biologique

Mots-clés géographiques Agrovoc : France

Mots-clés libres : Development, Gene network, Rice, Transcriptomic, Water deficit

Classification Agris : F60 - Physiologie et biochimie végétale
F30 - Génétique et amélioration des plantes
H50 - Troubles divers des plantes

Champ stratégique Cirad : CTS 2 (2019-) - Transitions agroécologiques

Agences de financement hors UE : Agropolis Fondation, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Empresa Brasileira de Pesquisa Agropecuária

Auteurs et affiliations

• Favreau Bénédicte, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0003-2786-9374 - auteur correspondant
• Gaal Camille, Université de Montpellier (FRA)
• Pereira De Lima Isabela, BASF (BRA)
• Droc Gaëtan, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0003-1849-1269
• Roques Sandrine, CIRAD-BIOS-UMR AGAP (FRA)
• Sotillo Armelle, CIRAD-BIOS-UMR AGAP (FRA)
• Guérard Florence, Université Paris-Saclay (FRA)
• Cantonny Valérie, Université Paris-Saclay (FRA)
• Gakière Bertrand, Université Paris-Saclay (FRA)
• Leclercq Julie, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0001-8110-3762
• Lafarge Tanguy, CIRAD-BIOS-UMR AGAP (FRA)
• De Raissac Marcel, CIRAD-BIOS-UMR AGAP (FRA)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/606538/)

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