Exploring the phytobeneficial and biocontrol capacities of endophytic bacteria isolated from hybrid vanilla pods

Lalanne-Tisné Guillaume, Barral Bastien, Taibi Ahmed, Kpatolo Coulibaly Zana, Burguet Pierre, Rasoarahona Felah, Quinton loic, Meile Jean-Christophe, Boubakri Hasna, Kodja Hippolyte. 2023. Exploring the phytobeneficial and biocontrol capacities of endophytic bacteria isolated from hybrid vanilla pods. Microorganisms, 11 (7):1754, 18 p.

Url - jeu de données - Entrepôt autre : https://www.ncbi.nlm.nih.gov/gene/77080708

Résumé : In this study, 58 endophytic bacterial strains were isolated from pods of two hybrid vanilla plants from Madagascar, Manitra ampotony and Tsy taitra. They were genetically characterized and divided into four distinct phylotypes. Three were associated to genus Bacillus species, and the fourth to the genus Curtobacterium. A selection of twelve strains corresponding to the identified genetic diversity were tested in vitro for four phytobeneficial capacities: phosphate solubilisation, free nitrogen fixation, and phytohormone and siderophore production. They were also evaluated in vitro for their ability to biocontrol the growth of the vanilla pathogenic fungi, Fusarium oxysporum f. sp. radicis vanillae and Cholletotrichum orchidophilum. Three bacteria of phylotype 4, m62a, m64 and m65, showed a high nitrogen fixation capacity in vitro, similar to the Pseudomonas florescens F113 bacterium used as a control (phospate solubilizing efficiency respectively 0.50 ± 0.07, 0.43 ± 0.07 and 0.40 ± 0.06 against 0.48 ± 0.03). Strain t2 related to B. subtilis showed a higher siderophore production than F113 (respectively 1.40 ± 0.1 AU and 1.2 ± 0.1 AU). The strain m72, associated with phylotype 2, showed the highest rate of production of Indole-3-acetic acid (IAA) in vitro. Bacteria belonging to the pylotype 4 showed the best capacity to inhibit fungal growth, especially the strains m62b m64 and t24, which also induced a significant zone of inhibition, suggesting that they may be good candidates for controlling fungal diseases of vanilla. This competence was highlighted with spectral imaging showing the production of lipopeptides (Iturin A2 and A3, C16 and C15-Fengycin A and C14 and C15-Surfactin) by the bacterial strains m65 confronted with the pathogenic fungi of vanilla.

Mots-clés Agrovoc : Fusarium oxysporum, Vanilla planifolia, lutte biologique, Fixation de l'azote, Vanilla, endophyte, champignon pathogène, Fusarium, Bacteria, maladie fongique

Mots-clés géographiques Agrovoc : France, Madagascar, La Réunion

Mots-clés libres : Vanilla endophytic bacteria, Plant microbiology, PGPB, Biocontrol activities, Spectral imaging

Classification Agris : H01 - Protection des végétaux - Considérations générales
U60 - Sciences de la vie et de la Terre

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Agences de financement européennes : European Regional Development Fund, European Commission

Programme de financement européen : H2020

Projets sur financement : (EU) EURLipids Interreg Eurogio Meuse-Rhine

Auteurs et affiliations

• Lalanne-Tisné Guillaume, Université de Montpellier (FRA)
• Barral Bastien, CIRAD-PERSYST-UMR Qualisud (FRA)
• Taibi Ahmed, Université de Montpellier (FRA)
• Kpatolo Coulibaly Zana, Université de Montpellier (FRA)
• Burguet Pierre, Université de la Réunion (REU)
• Rasoarahona Felah, Université d'Antananarivo (MDG)
• Quinton loic, Université de Liège (BEL)
• Meile Jean-Christophe, CIRAD-PERSYST-UMR Qualisud (FRA) ORCID: 0000-0002-6747-2104
• Boubakri Hasna, Université Claude Bernard (FRA)
• Kodja Hippolyte, Université de la Réunion (REU) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/608393/)

Métriques

[ Page générée et mise en cache le 2024-04-21 ]