Metabolite variations during the first weeks of growth of immature Citrus sinensis and Citrus reticulata by untargeted liquid chromatography–mass spectrometry/mass spectrometry metabolomics

Deschamps Estelle, Durand-Hulak Marie, Castagnos Denis, Hubert-Roux Marie, Schmitz Isabelle, Froelicher Yann, Afonso Carlos. 2024. Metabolite variations during the first weeks of growth of immature Citrus sinensis and Citrus reticulata by untargeted liquid chromatography–mass spectrometry/mass spectrometry metabolomics. Molecules, 29 (16):3718, 13 p.

Résumé : Immature citruses are an important resource for the pharmaceutical industry due to their high levels of metabolites with health benefits. In this study, we used untargeted liquid chromatography–mass spectrometry (LC-MS) metabolomics to investigate the changes associated with fruit size in immature citrus fruits in the first weeks of growth. Three orange cultivars (Citrus sinensis 'Navel', Citrus sinensis 'Valencia', and Citrus sinensis 'Valencia Late') and a mandarin (Citrus reticulata Blanco 'Fremont') were separated into eight fruit sizes, extracted, and analyzed. Statistical analyses revealed a distinct separation between the mandarin and the oranges based on 56 metabolites, with an additional separation between the 'Navel' orange and the 'Valencia' and 'Valencia Late' oranges based on 21 metabolites. Then, metabolites that evolved significantly with fruit size growth were identified, including 40 up-regulated and 31 down-regulated metabolites. This study provides new insights into the metabolite modifications of immature Citrus sinensis and Citrus reticulata in the first weeks of growth and emphasizes the significance of including early sampled fruits in citrus maturation studies.

Mots-clés Agrovoc : Citrus sinensis, Citrus reticulata, Citrus, agrume, variété, mandarine, croissance

Mots-clés géographiques Agrovoc : France, Corse

Mots-clés libres : Immature, Citrus sinensis, Orange, Citrus reticulata, Mandarin, Metabolomics, Orbitrap, LC-MS/MS, Variety discrimination, Fruit development

Agences de financement européennes : European Regional Development Fund

Agences de financement hors UE : INSA Rouen Normandie, Normandie Université, Région Normandie, Centre National de la Recherche Scientifique, Université de Rouen Normandie, Institut Carnot I2C, Oril Industrie

Programme de financement européen : H2020

Projets sur financement : (FRA) Synthèse Organique : des molécules au vivant, (FRA) Synthesizing our future

Auteurs et affiliations

• Deschamps Estelle, Université de Rouen Normandie (FRA)
• Durand-Hulak Marie, Domaine de la Triballe (FRA)
• Castagnos Denis, Oril Industrie (FRA)
• Hubert-Roux Marie, Université de Rouen Normandie (FRA)
• Schmitz Isabelle, CNRS (FRA) - auteur correspondant
• Froelicher Yann, CIRAD-BIOS-UMR AGAP (FRA)
• Afonso Carlos, Université de Rouen Normandie (FRA) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/612891/)

Métriques

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