Analyses of the root-knot nematode (Meloidogyne graminicola) transcriptome during host infection Highlight specific gene expression profiling in resistant rice plants

Petitot Anne-Sophie, Dereeper Alexis, Da Silva Corinne, Guy Julie, Fernandez Diana. 2020. Analyses of the root-knot nematode (Meloidogyne graminicola) transcriptome during host infection Highlight specific gene expression profiling in resistant rice plants. Pathogens, 9 (8):644, 21 p.

https://doi.org/10.3390/pathogens9080644

Article de revue ; Article de recherche ; Article de revue à facteur d'impact Revue en libre accès total

Prévisualisation

Version publiée - Anglais
Sous licence

.
613381.pdf
Télécharger (3MB) | Prévisualisation

Quartile : Q2, Sujet : MICROBIOLOGY

Résumé : The plant-parasitic nematode Meloidogyne graminicola causes considerable damages to rice (Oryza sativa) culture. Resistance to M. graminicola in the related species Oryza glaberrima reduces root penetration by juveniles and stops further nematode development. M. graminicola genes expressed during O. sativa infection were previously characterized but no information is available about the molecular dialogue established with a resistant plant. We compared the M. graminicola transcriptomes of stage-two juveniles (J2s) before and during infection of susceptible or resistant rice. Among 36,121 M. graminicola genes surveyed, 367 were differentially expressed during infection of resistant or susceptible plants. Genes encoding cell wall-degrading enzymes, peptidases and neuropeptides were expressed for a longer time in resistant plants compared to susceptible plants. Conversely, genes related to nematode development were not activated in the resistant host. The majority of M. graminicola effector genes had similar expression patterns, whatever the host genotype. However, two venom allergen-like protein (VAP)-encoding genes were specifically induced in resistant plants and Mg-VAP1 silencing in J2s reduced their ability to colonize roots. This study highlighted that M. graminicola adapts its gene expression to the host susceptibility. Further investigation is required to assess the role of Mg-VAPs in the rice–nematode interaction.

Mots-clés Agrovoc : Oryza sativa, résistance aux maladies, expression des gènes, Meloidogyne graminicola, résistance génétique, Oryza glaberrima, neuropeptide, nématode des plantes, gène, mécanisme de défense, physiologie végétale, relation hôte parasite