The expression pattern of alfalfa Flavanone 3-hydroxylase promoter-gus fusion in Nicotiana benthamiana correlates with the presence of flavonoids detected in situ

Charrier Bénédicte, Le Roux Christine, Kondorosi Adam, Ratet Pascal. 1996. The expression pattern of alfalfa Flavanone 3-hydroxylase promoter-gus fusion in Nicotiana benthamiana correlates with the presence of flavonoids detected in situ. Plant Molecular Biology, 30 : 1153-1168.

Autre titre : Mode d'expression de la fusion du gène promoteur "gus" de la Flavanone 3-hydroxylase de la luzerne chez Nicotiana benthamiana. Corrélation avec la présence de flavonoïdes détectés in situ

Résumé : Flavanone 3-hydroxylase is an enzyme acting in the central part of the flavonoid biosynthesis pathway. It is generally encoded by a single gene and seems to have a key position for the regulation in this pathway. These two features make a single #f3h# promoter-gus fusion a suitable tool to study both the #f3h# expression and the regulation of this pathway. We present here the spatial and temporal analysis of the expression of an alfalfa #flavanone# 3-hydroxylase# (#f3h#) promoter-gus fusion introduced into #nicotiana benthamiana#. The #medicago sativa# (alfalfa) #f3h# promoter directed gus expression in flowers, stems, leaves and roots. In flowers, GUS activity was observed in pollen grains, in ovules, in ovary placenta and in the epidermis, medullary parenchyma, trichomes and second cortical cellular layer surrounding the vascular bundles of the peduncle. In stems, GUS activity was detected at the same places as in the peduncle except for the medullary parenchyma. In roots, we found GUS staining in root hairs, epidermis and in the vascular bundles of the elongated zone. Finally, in leaves, the #f3h# promoter expressed essentially in the stalk cells of the multicellular trichomes. The expression pattern of the #f3h-gus# fusion was correlated to the presence of flavonoids #in situ#. These data indicate that this construct can be very useful to study factors controlling the production of flavonoids.

Mots-clés Agrovoc : flavonoïde, mesure (activité), fusion de gènes, Nicotiana, expression des gènes, enzyme, fleur, tige, feuille, racine

Mots-clés complémentaires : GUS

Classification Agris : F30 - Génétique et amélioration des plantes

Auteurs et affiliations

• Charrier Bénédicte, CNRS (FRA)
• Le Roux Christine, CIRAD-FORET-PLANTATIONS FORESTIERES (FRA) ORCID: 0000-0003-0668-3589
• Kondorosi Adam, CNRS (FRA)
• Ratet Pascal, CNRS (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/390004/)

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